9V0U

GPR133-Gain-miniG13 complex

9V0U の概要

• エントリーDOI: 10.2210/pdb9v0u/pdb
• EMDBエントリー: 64672
• 分子名称: Adhesion G-protein coupled receptor D1, Guanine nucleotide-binding protein subunit alpha-13,Isoform 2 of Guanine nucleotide-binding protein subunit alpha-13, Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2, ... (4 entities in total)
• 機能のキーワード: gpcr, g12/g13, complex, stachel, membrane protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 138039.87

構造登録者

Xi, Y.,Pu, X.,Ping, Y. (登録日: 2025-05-19, 公開日: 2025-07-30)

主引用文献

Pu, X.,Xi, Y.T.,Wang, M.X.,Zhang, D.,Ping, Y.Q.,Xiao, P.,Sun, J.P.
Cryo-EM structural elucidation and molecular mechanism of the GPR133-G13 signaling complex.
Biochem.Biophys.Res.Commun., 777:152165-152165, 2025

PubMed Abstract: GPR133 is an adhesion-class G protein-coupled receptor (GPCR) that has recently been de-orphanized. Its functions are complex and multifaceted. While GPR133 is primarily recognized for coupling with the Gs subunit to mediate elevated intracellular cAMP levels, its potential engagement with alternative signaling pathways remains poorly characterized. In our experiments, we demonstrated that GPR133 exhibits constitutive self-activation via its Stachel sequence as an adhesion GPCR, enabling activation of downstream G13 signaling. We reconstituted the GPR133-GAIN-miniGα13 complex in vitro and resolved its cryo-electron microscopy structure at a resolution of 3.51 Å. Detailed structural comparisons between the GPR133-GAIN-miniGα13 complex and the previously resolved GPR133-CTF-Gs structure highlighted both conserved and different features. These findings provide critical insights into the signal transduction mechanisms of GPR133 and lay a foundation for targeted therapeutic strategies.

PubMed: 40570642
DOI: 10.1016/j.bbrc.2025.152165

実験手法

ELECTRON MICROSCOPY (3.51 Å)