9UTE

influx carrier apo form

9UTE の概要

• エントリーDOI: 10.2210/pdb9ute/pdb
• EMDBエントリー: 64490
• 分子名称: Auxin transporter protein 1 (1 entity in total)
• 機能のキーワード: hormone transporter, hormone
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 54105.52

構造登録者

Chen, H.,Jiang, D. (登録日: 2025-05-03, 公開日: 2025-12-24)

主引用文献

Chen, H.,Fan, J.,Chi, C.,Zhao, J.,Wu, D.,Lei, X.,Deng, X.W.,Jiang, D.
Structural basis of auxin recognition and transport by the plant influx carrier AUX1.
Mol Plant, 18:1284-1293, 2025

PubMed Abstract: Auxin regulates numerous aspects of plant growth and development, featuring polar auxin transport mediated by auxin efflux and influx carriers. AUX1 is the major auxin importer that actively takes up natural and synthetic auxins. However, the precise mechanisms underlying AUX1-mediated auxin recognition and transport remain elusive. Here, we present cryoelectron microscopy structures of Arabidopsis thaliana AUX1 in both apo and auxin-bound states, revealing the structural basis for auxin recognition. Structural analyses show that AUX1 assumes the LeuT-like fold in an inward-facing conformation and the auxin analog 2,4-D is recognized by polar residues located in the central cavity of AUX1. Furthermore, we identify a putative cation-binding site that contributes to stabilizing the inward-facing conformation. Interestingly, we reveal that His249 undergoes a substantial conformational shift, and its mutation completely abolishes transport activity, suggesting a crucial role for His249 in AUX1 gating. Collectively, this study provides a structural foundation for a deeper understanding of auxin influx by AUX1-like carriers.

PubMed: 40579814
DOI: 10.1016/j.molp.2025.06.015

実験手法

ELECTRON MICROSCOPY (3.26 Å)