9UGT

Structure of Butanol Dehydrogenase in Complex with ADP and Co

9UGT の概要

• エントリーDOI: 10.2210/pdb9ugt/pdb
• 分子名称: NADH-dependent butanol dehydrogenase A, COBALT (II) ION, ADENOSINE-5'-DIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: national butanol dehydrogenase, biosynthetic protein
• 由来する生物種: Fusobacterium nucleatum
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 43822.24

構造登録者

Bai, X.,Meng, D.,Nam, K.H.,Xu, Y.B. (登録日: 2025-04-13, 公開日: 2025-10-29, 最終更新日: 2026-04-08)

主引用文献

Bai, X.,Nam, K.H.,Xu, Y.
Structural flexibility of the nicotinamide group of NADH in butanol dehydrogenase YqdH from Fusobacterium nucleatum.
Plos One, 20:e0338369-e0338369, 2025

PubMed Abstract: Butanol dehydrogenases (BDHs) are NAD(P)H-dependent oxidoreductases that catalyze the reversible conversion of butanol to butyraldehyde. These enzymes play essential roles in microbial butanol fermentation and show significant potential for biofuel synthesis and bioremediation. The crystal structures of BDHs from Fusobacterium nucleatum and Thermotoga maritima have clarified cofactor recognition and proposed reaction mechanisms. However, their distinct cofactor-binding modes and conformational differences in the substrate-binding cleft remain poorly characterized. In this study, we report the crystal structure of Fusobacterium nucleatum butanol dehydrogenase YqdH (FnYqdH) in a partially NADH-bound state. Electron density map analysis showed stable binding of the adenosine and diphosphate groups of NADH to the nucleotide-binding domain of FnYqdH. Conversely, the nicotinamide group was not observed, indicating that it was in an unbound state. Structural comparisons of FnYqdH complexed with either partial ADP or NADH revealed that the adenosine group is stabilized by hydrogen bonds with Thr143, Thr187, and Val184. Nicotinamide group binding induces positional and conformational changes in the diphosphate group of NADH. A comparative analysis of FnYqdH and TmBDH proteins revealed distinct conformational differences between their nucleotide-binding and catalytic domains, including variations in their substrate-binding metal ion sites. In particular, amino acid sequence and structural analyses of the BDH family revealed significant variability in the residues responsible for metal ion binding. Based on the observed flexibility of the nicotinamide group of NADH and the open conformation of FnYqdH, a potential reaction mechanism of FnYqdH is proposed. These findings offer valuable insights into the cofactor and substrate recognition within the BDH protein family.

PubMed: 41406140
DOI: 10.1371/journal.pone.0338369

実験手法

X-RAY DIFFRACTION (2.72 Å)