9UFR

Crystal structure of L-asparaginase from Thermococcus Sibiricus double mutation D54G/T56Q

Summary for 9UFR

Entry DOI	10.2210/pdb9ufr/pdb
Descriptor	asparaginase, DI(HYDROXYETHYL)ETHER, GLYCEROL, ... (5 entities in total)
Functional Keywords	l-asparaginase, hyperthermophilic enzyme, thermo-l-asparaginase, tsai, point mutant, double mutation, hydrolase
Biological source	Thermococcus sibiricus
Total number of polymer chains	1
Total formula weight	36929.14
Authors	Matyuta, I.O.,Varfolomeeva, L.A.,Minyaev, M.E.,Dumina, M.,Zhdanov, D.,Zhgun, A.,Pokrovskaya, M.,Aleksandrova, S.,El'darov, M.,Popov, V.O.,Boyko, K.M. (deposition date: 2025-04-10, release date: 2025-06-11, Last modification date: 2025-06-25)
Primary citation	Dumina, M.V.,Zhdanov, D.D.,Veselovsky, A.V.,Pokrovskaya, M.V.,Aleksandrova, S.S.,Minyaev, M.E.,Varfolomeeva, L.A.,Matyuta, I.O.,Boyko, K.M.,Zhgun, A.A. Hyperthermophilic L-Asparaginase from Thermococcus sibiricus and Its Double Mutant with Increased Activity: Insights into Substrate Specificity and Structure. Int J Mol Sci, 26:-, 2025 Cited by DOI: 10.3390/ijms26125437 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.9 Å)

Structure validation