9UC3

Crystal structure of voltage-gated sodium channel NavAb N49K/S178G mutant

9UC3 の概要

• エントリーDOI: 10.2210/pdb9uc3/pdb
• 分子名称: Ion transport protein, CALCIUM ION, CHAPSO, ... (6 entities in total)
• 機能のキーワード: ion channel, membrane protein
• 由来する生物種: Aliarcobacter butzleri
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 38061.51

構造登録者

Irie, K.,Maeda, Y.,Kojima, K. (登録日: 2025-04-03, 公開日: 2026-02-18, 最終更新日: 2026-03-04)

主引用文献

Maeda, Y.K.,Kojima, K.,Nakamura, T.Y.,Nakatsu, T.,Irie, K.
Structure-function analysis of the lithium-ion selectivity of the voltage-gated sodium channel.
J.Gen.Physiol., 158:-, 2026

PubMed Abstract: Voltage-gated sodium channels (Navs) selectively conduct Na+ to generate action potentials. Na+ permeates Navs with significantly higher efficiency than many other cations, but Li+ can also permeate Navs to an extent comparable with Na+. Li+ in the blood is known to enter cells via Navs and to have a beneficial effect on various neuropathies. However, the molecular basis of the high Li+ selectivity of Navs was unclear. In this study, using a prokaryotic Nav, we successfully created the first Nav mutant to be more selective for Li+ than for Na+. Electrophysiological and crystallographic analyses suggested the critical determinants of high Li+ selectivity: the strong electrostatic interaction between the ion pathway and hydrated ions, and the smaller number of hydration water exchanges within the ion pathway. Additionally, the extensive interactions around the ion pathway were shown to support monovalent cation selectivity. New drug directions based on the molecular basis for Li+ permeation may target various neurological disorders and could clarify the broader biological effects of lithium.

PubMed: 41711638
DOI: 10.1085/jgp.202513855

実験手法

X-RAY DIFFRACTION (2.9 Å)