9U7M

Solution NMR structure of SA-XV peptide bound to SDS micelle.

9U7M の概要

• エントリーDOI: 10.2210/pdb9u7m/pdb
• 分子名称: Calcitermin (1 entity in total)
• 機能のキーワード: structure from cyana 2.1 antifungal peptide microbial keratitis random coil loosely bound to sds micelle, antimicrobial protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 1693.96

構造登録者

Biswas, K.,Bhunia, A.,Roy, S. (登録日: 2025-03-24, 公開日: 2025-09-24, 最終更新日: 2025-10-29)

主引用文献

Agarwal, R.,Biswas, K.,Agrawal, A.,Shankar, N.N.,Kundu, S.,Roy, D.,Son, D.,Harikishore, A.,Yennamalli, R.M.,Lee, D.,Bhunia, A.,Roy, S.
SA-XV, a 15-amino acid fragment of host defense peptide S100A12, targets mitochondria and is protective against fungal infections.
J.Biol.Chem., 301:110743-110743, 2025

PubMed Abstract: Fungal infections are huge emerging crisis with more than two million people infected worldwide annually. Corneal infections caused by fungus is the major cause of vision loss and often warrants corneal transplantation. Both Fusarium spp. and Candida spp. are critical etiological agents of fungal keratitis and also common cause for invasive fungal infections with high mortality rates. In previous work we described growth inhibition of Fusarium spp. by S100A12, a host antimicrobial peptide. Here, to optimize a potential therapeutic, we have studied a 15 amino acid fragment of S100A12, SA-XV. Interestingly, SA-XV demonstrated remarkable antifungal activities, similar to the parent peptide, against both Fusarium spp. and Candida spp. SA-XV is a cell penetrating peptide, and once internalized, it binds to fungal DNA, halts cell cycle, and disrupts mitochondria leading to generation of reactive oxygen species and cell damage. Atomistic structure of the peptide determined by NMR reveals that SA-XV associates with fungal membrane. The structural changes in SA-XV from α-helical to random coil conformation was observed in all-atom simulations. Additionally, SA-XV aids in wound healing of corneal epithelial cells and attenuate the fungal burden in a murine model of fungal keratitis. Our results clearly demonstrate SA-XV as a promising antifungal candidate that targets both filamentous and non-filamentous fungus for alternative therapeutic interventions.

PubMed: 40975173
DOI: 10.1016/j.jbc.2025.110743

実験手法

SOLUTION NMR