9U7A

FADD-DED filaments coordinate complex IIa assembly during TNF-induced apoptosis

9U7A の概要

• エントリーDOI: 10.2210/pdb9u7a/pdb
• EMDBエントリー: 63933
• 分子名称: FAS-associated death domain protein (1 entity in total)
• 機能のキーワード: rna recognition, rna helicase, atp hydrolysis, rlr signaling, immune system
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 24
• 化学式量合計: 244841.33

構造登録者

Liu, P.,Luo, D. (登録日: 2025-03-24, 公開日: 2025-08-20, 最終更新日: 2025-09-03)

主引用文献

Chen, Y.,Huynh, V.T.,Lai, L.,Liu, P.,Li, T.,Tan, Y.B.,Chew, C.S.,Velazquez, A.M.V.,Samsudin, F.,Marzinek, J.K.,Bond, P.J.,Wu, B.,Luo, D.,Tergaonkar, V.
FADD DED filaments coordinate complex IIa assembly during TNF-induced apoptosis.
Proc.Natl.Acad.Sci.USA, 122:e2425802122-e2425802122, 2025

PubMed Abstract: Extrinsic apoptosis is initiated by signaling from death receptors, leading to the assembly of RIPK1, FADD, and caspase-8 complex. Subsequently, caspase-8 forms a filamentous structure through the oligomerization of its tandem death effector domain (tDED), resulting in caspase activation and cell death. Although the DED of FADD (DED) is homologous to the tDEDs of caspase-8 (tDED) and both oligomerize to function, the functional form of DED oligomer in extrinsic apoptosis remains unclear. Here, using cryogenic-electron microscopy, we elucidate the structure of DED filaments comprising three helical chains assembled through three types of iterative interactions. Mutations disrupting DED filament formation impair the recruitment of RIPK1 and caspase-8, and abrogate the cell death response, suggesting that DED filamentation represents an important mechanistic step in the initiation of TNF-induced extrinsic apoptosis. Contrary to the belief that the homotypic death domains of RIPK1 and FADD are solely responsible for their interaction, we here show this interaction requires DED filamentation. Furthermore, cFLIP can disrupt DED filaments, uncovering an additional antiapoptotic mechanism of cFLIP beyond its disruption of caspase-8 filament. Molecular dynamics simulations reveal that DED filament thermodynamically favors tDED monomer over DED monomer, thus explaining the hierarchy and stoichiometry of FADD/caspase-8 complex assembly. These findings highlight the hitherto unappreciated roles of DED filament formation in extrinsic apoptosis.

PubMed: 40838879
DOI: 10.1073/pnas.2425802122

実験手法

ELECTRON MICROSCOPY (2.82 Å)