9TP0

CO dehydrogenase 2 variant A559W V610H

9TP0 の概要

• エントリーDOI: 10.2210/pdb9tp0/pdb
• 分子名称: Carbon monoxide dehydrogenase 2, IRON/SULFUR CLUSTER, FE2/S2 (INORGANIC) CLUSTER, ... (6 entities in total)
• 機能のキーワード: codh, channel, a559w, cluster c, 4fe-4s, iron, iron-sulfur, nickel, oxidoreductase
• 由来する生物種: Carboxydothermus hydrogenoformans Z-2901
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 67981.24

構造登録者

Gebhardt, P.,Dobbek, H.,Jeoung, J.-H. (登録日: 2025-12-17, 公開日: 2026-06-03)

主引用文献

Opdam, L.V.,Gebhardt, P.,Leger, C.,Dobbek, H.,Fourmond, V.
Correspondence on "Fortification of FeS Clusters Reshapes Anaerobic CO Dehydrogenase Into an Air-Viable Enzyme Through Multilayered Sealing of O 2 Tunnels".
Angew.Chem.Int.Ed.Engl., :e1942100-e1942100, 2026

PubMed Abstract: In their recent communication in Angewandte Chemie (10.1002/anie.202508565), Suk Min Kim and coworkers have described the effect of modifying the gas channels of the CO dehydrogenase II from Carboxydothermus hydrogenoformans, an enzyme that oxidizes reversibly CO into CO. Their goal was to use mutagenesis to slow down the arrival of O at the active site. They reported a large increase in the resistance against oxygen, one of the major barriers to the application of this extremely fast and efficient enzyme in biotechnological devices, with an increase in the IC of more than two orders of magnitudes for some variants, with only a minor impact on the affinity of the enzyme for CO. We have produced the same variants, and characterized them in depth using Protein Film Electrochemistry. We used an approach that has proven very useful to learn and understand about the reactivity of CO dehydrogenases (and other redox enzymes like hydrogenases) with O. We found that, contrary to the claims by Kim and coworkers, the A559W and the A559W/V610H mutants are not more resistant than the wild type against oxygen.

PubMed: 42175862
DOI: 10.1002/anie.1942100

実験手法

X-RAY DIFFRACTION (1.41 Å)