9TM7

Unspecific Peroxygenase from Daldinia childiae

9TM7 の概要

• エントリーDOI: 10.2210/pdb9tm7/pdb
• 分子名称: Unspecific Peroxygenase, MAGNESIUM ION, PROTOPORPHYRIN IX CONTAINING FE, ... (4 entities in total)
• 機能のキーワード: oxidoreductase, heme, unspecific peroxygenase
• 由来する生物種: Daldinia childiae
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 62484.05

構造登録者

McKenzie, A.,Clark, C.,Melling, B.,Domenech, J.,Grogan, G. (登録日: 2025-12-12, 公開日: 2026-07-29)

主引用文献

McKenzie, A.,Clark, C.,Cornish, K.A.S.,Li, J.,Domenech, J.,Melling, B.,Ralston, M.P.H.,Cartwright, J.,Mulholland, N.P.,Unsworth, W.P.,Grogan, G.
Structure, characterisation and application of an unspecific peroxygenase from Daldinia childiae.
Rsc Chem Biol, 2026

PubMed Abstract: Unspecific peroxygenases (UPOs) have emerged as useful biocatalysts for the scalable and selective oxygenation of a large variety of organic molecules. UPOs have been divided into family I and family II enzymes, dependent upon sequence similarity and molecular weight, with family I being shorter in sequence. Here we report the characterisation and application of the family I UPO from (UPO). The enzyme was expressed in both and , yielding protein for kinetic and structural studies and biocatalytic application respectively. The structure of the enzyme revealed notable differences in the active site tunnel, compared with the well-studied family I artUPO, including F79 for V69 and F171 for I160. Notably, these differences were manifested in selectivity divergent from other UPOs when UPO was applied to preparative biotransformations; for example, (-)-menthol was converted exclusively into -6-hydroxymenthol in contrast to artUPO, which gave exclusively the tertiary alcohol 2,8-menthanediol.

PubMed: 42328014
DOI: 10.1039/d6cb00141f

実験手法

X-RAY DIFFRACTION (1.88 Å)