9SXC

Crystal structure of the Legionella pneumophila effector SidL (Lpg0437) in complex with its metaeffector LegA11 (Lpg0436)

9SXC の概要

• エントリーDOI: 10.2210/pdb9sxc/pdb
• 分子名称: Dot/Icm secretion system substrate, Ankyrin repeat-containing protein (3 entities in total)
• 機能のキーワード: legionella, effector, hydrolase
• 由来する生物種: Legionella pneumophila; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 102026.72

構造登録者

Machtens, D.A.,Willerding, J.M.,Eschenburg, S.,Reubold, T.F. (登録日: 2025-10-09, 公開日: 2025-12-10)

主引用文献

Machtens, D.A.,Hutchison, C.A.,Stein, A.M.,Eberhage, J.,Willerding, J.M.,Eschenburg, S.,Shames, S.R.,Reubold, T.F.
Crystal structure of the Legionella pneumophila effector SidL (Lpg0437) in complex with its metaeffector LegA11 (Lpg0436).
Biorxiv, 2025

PubMed Abstract: is an opportunistic human pathogen that causes atypical pneumonia called Legionnaires' Disease. To replicate within host cells, injects up to 330 effector proteins into the host cytosol via a Dot/Icm type IV secretion system. Several effectors, called metaeffectors, regulate the activity of other effectors within infected host cells through direct protein-protein interactions. LegA11 (AnkJ/Lpg0436) has been identified as a putative metaeffector of SidL (Ceg14/Lpg0437), one of eight effectors that inhibit host mRNA translation. LegA11 binds and suppresses SidL enzymatic activity, but the molecular basis of this regulation and impact on mRNA translation are unknown. Here, we present the crystal structure of SidL in complex with LegA11 to a resolution of 2.4 Å, revealing a high-affinity 1:1 complex with an extensive interaction interface of ~2300 Å. Using isothermal titration calorimetry, we determined a dissociation constant of 1.8 nM. translation assays demonstrate that SidL inhibits mRNA translation, and this activity is completely suppressed by LegA11. Mutagenesis of key interface residues in LegA11 disrupts complex formation and abolishes its metaeffector activity, confirming that LegA11 regulates SidL through direct protein-protein interaction. These findings establish LegA11 as a metaeffector that contributes to suppression of host mRNA translation by .

PubMed: 41278709
DOI: 10.1101/2025.10.23.683979

実験手法

X-RAY DIFFRACTION (2.4 Å)