9SRC

Cryo-EM structure of P. abyssi 70S ribosome in complex with hibernation factor HibA in PTC conformation

これはPDB形式変換不可エントリーです。

9SRC の概要

• エントリーDOI: 10.2210/pdb9src/pdb
• EMDBエントリー: 55137
• 分子名称: rRNA 23S, 30S ribosomal protein S5, 30S ribosomal protein S6e, ... (67 entities in total)
• 機能のキーワード: 70s, ribosome, hibernation, pyrococcus abyssi
• 由来する生物種: Pyrococcus abyssi GE5; 詳細
• タンパク質・核酸の鎖数: 69
• 化学式量合計: 2541080.83

構造登録者

Madru, C.M.,Bourgeois, G.B.,Mechulam, Y.M.,Schmitt, E.S. (登録日: 2025-09-24, 公開日: 2026-07-22, 最終更新日: 2026-08-12)

主引用文献

Madru, C.,Bourgeois, G.,Dulermo, R.,Capeyrou, R.,Joncour, G.,Figuigui, K.,Duchateau, M.,Chamot-Rooke, J.,Duboc, C.,l'Haridon, S.,Mc Teer, L.,Kwapisz, M.,Clouet-d'Orval, B.,Bouvier, M.,Mechulam, Y.,Borrel, G.,Schmitt, E.,Flament, D.
A family of ribosome hibernation factors widespread in Archaea.
Nat Commun, 17:-, 2026

PubMed Abstract: Ribosome hibernation preserves translation machinery during stress, yet its mechanisms in Archaea remain poorly defined. Using cryo-EM analysis, we studied hibernation pathways in Pyrococcus abyssi stressed cells. We identified HibA, a previously unrecognized family of hibernation factors widespread in Archaea. HibA consists of a bacterial-like HPF/RaiA domain fused to a Cystathionine Beta Synthase module. Unexpectedly, HibA binds to the ribosome in three different conformations, occupying the A, P and E sites of tRNAs, as well as that of mRNA, enhancing its ability to protect the ribosome from degradation. Idle ribosomes also frequently accumulate the archaeal homolog of eukaryotic ribosome maturation protein SBDS (aSBDS), suggesting that stressed archaeal cells may engage parallel hibernation routes in which aSBDS can complement HibA. Deletion of hibA in Thermococcus barophilus delays recovery from stationary phase and reduces 70S ribosome pools, establishing its role in ribosome preservation. Taxonomic profiling shows that many archaeal lineages encode distinct repertoires of ribosome-associated protection factors, underscoring the modular and multi-layered nature of archaeal hibernation systems. In addition, a comprehensive phylogenetic analysis highlights the evolutionary relationships between prevalent ribosome hibernation factors across Bacteria and Archaea.

PubMed: 42045235
DOI: 10.1038/s41467-026-72341-8

実験手法

ELECTRON MICROSCOPY (2.1 Å)