9SNT

Apo-form of Schistosoma mansoni Cathepsin D1 at 2.2A resolution

9SNT の概要

• エントリーDOI: 10.2210/pdb9snt/pdb
• 関連するPDBエントリー: 9RXI
• 分子名称: Cathepsin D (A01 family) (2 entities in total)
• 機能のキーワード: apo-form, cathepsin d, lysosomal aspartyl protease, hydrolase
• 由来する生物種: Schistosoma mansoni
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 47001.06

構造登録者

Parker, K.,Clarke, J.D.,Liu, X.,Gomes, B.F.,Eyssen, L.E.,Furnham, N.,Silva-Jr, F.P.,Owens, R.J. (登録日: 2025-09-11, 公開日: 2026-02-18)

主引用文献

Parker, K.L.,Clarke, J.D.,Liu, X.,Gomes, B.F.,Eyssen, L.E.A.,Furnham, N.,Paes Silva-Jr, F.,Owens, R.J.
Crystal structure of Schistosoma mansoni cathepsin D1 in complex with a nanobody reveals the conformation of the propeptide-bound state.
Acta Crystallogr D Struct Biol, 82:140-150, 2026

PubMed Abstract: Schistosoma mansoni cathepsin D1 (SmCD1) has been shown to be an essential enzyme for helminth metabolism due to its role in haemoglobin degradation: a key amino-acid source for the developing parasite. Therefore, the enzyme is a potential target for the development of antischistosomal inhibitors. SmCD1 has significant sequence identity to cathepsin D-like proteases found in other schistosome species and homology to mammalian aspartic proteases. Here, we report the first crystal structures of a helminth cathepsin D, SmCD1, and have identified a single-domain antibody (nanobody) that specifically binds to SmCD1 with nanomolar affinity but does not recognize human cathepsin D. We have mapped the epitope of the nanobody by determining the crystal structure of the enzyme-nanobody complex, revealing the conformation of SmCD1 in the propeptide-bound state.

PubMed: 41603320
DOI: 10.1107/S2059798326000422

実験手法

X-RAY DIFFRACTION (2.2 Å)