9SJ5

Chromosomal Passenger Complex in complex with H3T3ph Nucleosome (Class1)

9SJ5 の概要

• エントリーDOI: 10.2210/pdb9sj5/pdb
• 関連するPDBエントリー: 9SI3
• EMDBエントリー: 54938
• 分子名称: Histone H3.2, Inner centromere protein, DNA (147-MER), ... (10 entities in total)
• 機能のキーワード: nucleosome-binding dna-binding stabilising nucleosome acidic-patch interaction, cell cycle
• 由来する生物種: Xenopus laevis (African clawed frog); 詳細
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 384340.03

構造登録者

Gireesh, A.,Abad, M.A.,Sotelo-Parrilla, P.,Jeyaprakash, A.A. (登録日: 2025-08-29, 公開日: 2025-10-01, 最終更新日: 2025-11-26)

主引用文献

Gireesh, A.,Abad, M.A.,Nozawa, R.S.,Sotelo-Parrilla, P.,Dury, L.C.,Likhodeeva, M.,Wear, M.,Spanos, C.,Peralta, C.C.,Rappsilber, J.,Hopfner, K.P.,Wilson, M.D.,Vanderlinden, W.,Hirota, T.,Jeyaprakash, A.A.
Nucleosome interaction of the CPC secures centromeric chromatin integrity and chromosome segregation fidelity.
Embo J., 44:6556-6597, 2025

PubMed Abstract: The chromosomal passenger complex (CPC; Borealin-Survivin-INCENP-Aurora B kinase) ensures accurate chromosome segregation by orchestrating sister chromatid cohesion, error correction of kinetochore-microtubule attachments, and spindle assembly checkpoint signaling. Correct spatiotemporal regulation of CPC is critical for its function. Phosphorylations of histone H3 Thr3 and histone H2A Thr120 and modification-independent nucleosome interactions involving Survivin and Borealin contribute to CPC centromere enrichment. However, how various nucleosome binding elements collectively contribute to CPC centromere enrichment at the mechanistic level, and whether CPC has any non-catalytic role at centromere remain open questions. Combining the high-resolution cryo-EM structure of a CPC-bound H3Thr3ph nucleosome with atomic force microscopy and biochemical and cellular assays, we demonstrate that CPC employs multipartite interactions, which facilitate its engagement with nucleosome acidic patch and the DNA entry-exit site. Perturbing the CPC-nucleosome interaction compromises chromatin protection against MNase digestion in vitro, and centromeric chromatin stability and error-free chromosome segregation in cells. Our work suggests a non-catalytic chromatin-stabilizing role of CPC in maintaining centromeric chromatin features critical for kinetochore function.

PubMed: 41145915
DOI: 10.1038/s44318-025-00594-y

実験手法

ELECTRON MICROSCOPY (2.85 Å)