9RKU

Agrobacterium phage 7-7-1 baseplate

これはPDB形式変換不可エントリーです。

9RKU の概要

• エントリーDOI: 10.2210/pdb9rku/pdb
• EMDBエントリー: 54021
• 分子名称: Baseplate protein, Putative tail biosynthetic protein, Structural protein, ... (8 entities in total)
• 機能のキーワード: flagella phage, agrobacterium sp. h13-3, baseplate, virus
• 由来する生物種: Agrobacterium phage 7-7-1; 詳細
• タンパク質・核酸の鎖数: 60
• 化学式量合計: 2122376.62

構造登録者

Noteborn, W.E.M.,Hoeksma, T.,Briegel, A. (登録日: 2025-06-14, 公開日: 2025-12-10, 最終更新日: 2026-01-21)

主引用文献

Noteborn, W.E.M.,Ouyang, R.,Hoeksma, T.,Sidi Mabrouk, A.,Esteves, N.C.,Pelt, D.M.,Scharf, B.E.,Briegel, A.
Insights into the structure and initial host attachment of the flagellotropic bacteriophage 7-7-1.
Commun Biol, 9:55-55, 2025

PubMed Abstract: Understanding the structural and functional mechanisms of bacteriophage 7-7-1, the flagellotropic phage infecting Agrobacterium sp. H13-3, offers promising insights into phage-host interactions. Using single particle analysis (SPA) cryo-electron microscopy (cryo-EM), we determined the capsid, neck region, tail, and baseplate complex structures. Combined with cryo-electron tomography (cryo-ET) and machine learning methodologies, our findings indicate that phage 7-7-1 uses capsid fibers to establish initial contact with the host flagellum, followed by subsequent attachment to cell surface receptors. The study also demonstrated that capsid fibers are flexible and can interact with other phages and host flagella, suggesting a cooperative infection strategy. These results provide crucial structural insights and may open avenues for developing phage-based therapeutics against resistant bacterial pathogens.

PubMed: 41353512
DOI: 10.1038/s42003-025-09319-7

実験手法

ELECTRON MICROSCOPY (3.44 Å)