9RHL

Phospho-DH bound by Sld3-MBD on ARS1 DNA

これはPDB形式変換不可エントリーです。

9RHL の概要

• エントリーDOI: 10.2210/pdb9rhl/pdb
• EMDBエントリー: 53972
• 分子名称: DNA replication licensing factor MCM2, ZINC ION, ADENOSINE-5'-DIPHOSPHATE, ... (12 entities in total)
• 機能のキーワード: macromolecular complex, dna, atpase, helicase, mcm2-7, replication
• 由来する生物種: Saccharomyces cerevisiae (brewer's yeast); 詳細
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 1418475.31

構造登録者

Puehringer, T.,Couves, E.C.,Costa, A. (登録日: 2025-06-09, 公開日: 2026-05-13, 最終更新日: 2026-07-01)

主引用文献

Puhringer, T.,Canal, B.,Palm, G.,Butryn, A.,Couves, E.C.,Willhoft, O.,Lewis, J.S.,Diffley, J.F.X.,Costa, A.
Structure of the pre-initiation complex explains CMGE biogenesis.
Nature, 2026

PubMed Abstract: When cells enter S phase, bidirectional DNA replication is initiated through the kinase-regulated recruitment of three activators (Cdc45, GINS and Pol ε) to a duplex-DNA-loaded double hexamer of minichromosome maintenance (MCM) ATPases. Together, these proteins form two CMGE helicases that establish divergent replication forks as they become separated. Here, to gain an understanding of CMGE biogenesis, we reconstituted the pre-initiation complex with purified yeast proteins. The cryo-electron-microscopy structure shows a set of firing factors caught in the act of assembling two symmetrical CMGEs. We show how stepwise complex formation reshapes MCM in preparation for DNA opening, and we explain how ATP promotes firing-factor ejection and CMGE maturation. We find that although Sld2 facilitates the recruitment of GINS to MCM, as expected, it also aids the efficient separation of the CMGE dimer, and is essential for the ejection of the lagging strand from MCM. These findings have direct implications for our understanding of the metazoan Sld2 orthologue, RECQL4, and point to a replication-fork establishment mechanism that is conserved across eukaryotes.

PubMed: 42310460
DOI: 10.1038/s41586-026-10657-7

実験手法

ELECTRON MICROSCOPY (3.1 Å)