9RCE

Cryo-EM structure of a contractile injection system in Salmonella enterica subspecies Salamae, the baseplate portion in extended state.

これはPDB形式変換不可エントリーです。

9RCE の概要

• エントリーDOI: 10.2210/pdb9rce/pdb
• EMDBエントリー: 53919
• 分子名称: Peptidoglycan-binding protein, Conserved hypothetical phage tail region protein, SalCis12, ... (9 entities in total)
• 機能のキーワード: contractile injection system salmonella, contractile protein
• 由来する生物種: Salmonella enterica subsp. salamae; 詳細
• タンパク質・核酸の鎖数: 15
• 化学式量合計: 797626.75

構造登録者

Ejaz, R.N.,Tillmann, H.P.,Sofos, N.H.,Siborova, M.,Taylor, N.M.I. (登録日: 2025-05-28, 公開日: 2026-04-22, 最終更新日: 2026-05-06)

主引用文献

Ejaz, R.N.,Funke, K.,Kielkopf, C.S.,Martin, F.J.O.,Siborova, M.,Hendriks, I.A.,Sofos, N.H.,Pape, T.,Steiner-Rebrova, E.M.,Nielsen, M.L.,Erhardt, M.,Taylor, N.M.I.
Structure of a contractile injection system in Salmonella enterica subsp. salamae.
Nat Commun, 2026

PubMed Abstract: Extracellular contractile injection systems (eCISs) are phage-derived nanomachines used by bacteria to deliver effectors into target cells. Well-studied examples include the Photorhabdus asymbiotica virulence cassettes and the antifeeding prophage from Serratia entomophila, which have been engineered for heterologous cargo delivery. Recent genomic analyses identified eCIS gene clusters in the opportunistic human pathogen Salmonella enterica subspecies salamae, but their structure, function, and biotechnological potential remain unexplored. Here, we report a high-resolution cryo-electron microscopy structure of the S. enterica eCIS. Our atomic models reveal a distinctive sheath architecture, an expansive cage-like shell around a central spike, and an associated integral membrane protein. We identify a putative effector encoded within the operon exhibiting mild periplasmic toxicity and provide evidence that the S. enterica eCIS deviates from canonical eCISs by interacting with the inner membrane. Guided by these structural features, we uncover, to the best of our knowledge, a previously unannotated cluster of contractile injection systems (CISs). Together, our findings expand the known diversity of CISs' structures and functions, and lay the groundwork for engineering customisable protein delivery platforms.

PubMed: 41991534
DOI: 10.1038/s41467-026-71989-6

実験手法

ELECTRON MICROSCOPY (2.86 Å)