9RAW

The L1 amyloid-beta(1-40)fibril in the presence of anle138b (post-treatment)

9RAW の概要

• エントリーDOI: 10.2210/pdb9raw/pdb
• EMDBエントリー: 53880
• 分子名称: Amyloid-beta A4 protein (1 entity in total)
• 機能のキーワード: amyloid-beta, fibril, anle138b, protein fibril
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 43358.52

構造登録者

Frieg, B.,Han, M.,Griesinger, C.,Schroeder, G.F. (登録日: 2025-05-21, 公開日: 2025-10-15)

主引用文献

Han, M.,Frieg, B.,Matthes, D.,Leonov, A.,Ryazanov, S.,Giller, K.,Nimerovsky, E.,Stampolaki, M.,Xue, K.,Overkamp, K.,Dienemann, C.,Riedel, D.,Giese, A.,Becker, S.,de Groot, B.L.,Schroder, G.F.,Andreas, L.B.,Griesinger, C.
Anle138b binds predominantly to the central cavity in lipidic A beta 40 fibrils and modulates fibril formation.
Nat Commun, 16:8850-8850, 2025

PubMed Abstract: Alzheimer's disease is a specific neurodegenerative disorder, distinct from normal aging, with a growing unmet medical need. It is characterized by the accumulation of amyloid plaques in the brain, primarily consisting of amyloid beta (Aβ) fibrils. Therapeutic antibodies can slow down the disease, but are associated with potential severe side effects, motivating the development of small molecules to halt disease progression. This study investigates the interaction between the clinical drug candidate small molecule anle138b and lipidic Aβ₄₀ fibrils of type 1 (L1). L1 fibrils were previously shown to closely resemble fibrils from Alzheimer's patients. Using high-resolution structural biology techniques, including cryo-electron microscopy (cryo-EM), nuclear magnetic resonance (NMR) spectroscopy enhanced by dynamic nuclear polarization (DNP), and molecular dynamics (MD) simulations, we find that anle138b selectively binds to a cavity within the fibril. This structural insight provides a deeper understanding of a potential drug-binding mechanism at the atomic level and may inform the development of therapies and diagnostic approaches. In addition, anle138b reduces fibril formation in the presence of lipids by approximately 75%. This may suggest a mechanistic connection to its previously reported activity in animal models of Alzheimer's disease.

PubMed: 41044155
DOI: 10.1038/s41467-025-64443-6

実験手法

ELECTRON MICROSCOPY (2.79 Å)