9R4K

Crystal structure of CtGH76 from Chaetomium thermophilum

9R4K の概要

• エントリーDOI: 10.2210/pdb9r4k/pdb
• 分子名称: Chains: A, GLYCEROL, FORMIC ACID, ... (5 entities in total)
• 機能のキーワード: glycoside hydrolases, carbohydrate-binding protein, hydrolase
• 由来する生物種: Thermochaetoides thermophila DSM 1495
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 48526.47

構造登録者

Po-Hsun, W.,Essen, L.-O. (登録日: 2025-05-07, 公開日: 2025-07-16, 最終更新日: 2025-08-06)

主引用文献

Ruppenthal, S.R.,Po-Hsun, W.,Watad, M.,Rosner, C.J.,Vogt, M.S.,Friedrich, M.,Voigt, A.L.,Petz, A.,Gnau, P.,Essen, L.O.
Ct GH76, a Glycoside Hydrolase 76 from Chaetomium thermophilum , with Elongated Glycan-Binding Canyon.
Int J Mol Sci, 26:-, 2025

PubMed Abstract: Fungal cell walls, composed of polysaccharides and proteins, play critical roles in adaptation, cell division, and protection against environmental stress. Their polyglucan components are continuously remodeled by various types of glycosyl hydrolases (GHs) and transferases (GTs). In and other ascomycetes, enzymes of the subfamily, which belong as GTs to the GH76 family, cleave an α1,4 linkage between glucosamine and mannose to facilitate covalent linkage of GPI-anchored proteins to the cell wall's polyglucans. In contrast, the functions of other fungal GH76 subfamilies are not understood. We characterized GH76 from the sordariomycete , a member of the GH76 subfamily, revealing conserved structural features and functional divergence within the GH76 family. Notably, our structural characterization by X-ray crystallography combined with glycan fragment screening indicated that GH76 can recognize GPI-anchors like members of the subfamily but shows a broader promiscuity toward other glycans with central α1,6-mannobiose motifs due to the presence of an elongated glycan-binding . These findings provide new insights into GH76 enzyme diversity and fungal cell wall maturation.

PubMed: 40724838
DOI: 10.3390/ijms26146589

実験手法

X-RAY DIFFRACTION (2.02 Å)