9R4E

Spitrobot-2 advances time-resolvedcryo-trapping crystallography to under 25 ms: Human insulin, pH 4.5 (5 s soaking)

9R4E の概要

• エントリーDOI: 10.2210/pdb9r4e/pdb
• 関連するPDBエントリー: 1b18
• 分子名称: Insulin A chain, Insulin B chain, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: human insulin, ph-jump, hormone
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 5913.71

構造登録者

Spiliopoulou, M.,Hatton, C.E.,Mehrabi, P.,Schulz, E.C. (登録日: 2025-05-07, 公開日: 2025-12-03)

主引用文献

Spiliopoulou, M.,Hatton, C.E.,Kollewe, M.,Leimkohl, J.P.,Schikora, H.,Tellkamp, F.,Mehrabi, P.,Schulz, E.C.
Spitrobot-2 advances time-resolved cryo-trapping crystallography to under 25 ms.
Commun Chem, 8:363-363, 2025

PubMed Abstract: We previously introduced the spitrobot, a protein crystal plunging system that enables reaction quenching via cryo-trapping with a time resolution in the millisecond range. Here we present the next generation, spitrobot-2, as an integrated benchtop device. User-friendliness has been improved by semi-automatic sample exchange. Moreover, a fully automated shutter shields the liquid nitrogen from the humidified environment, improving sample integrity. Most importantly, the cryo-trapping delay time has been reduced to 23 ms, making spitrobot-2 twice as fast as the previous generation. This further expands the number of target systems that can be addressed by cryo-trapping time-resolved crystallography. Using 12 crystal structures of three independent model systems, we demonstrate successful cryo-trapping via observation of conformational changes and ligand binding within 25 ms. These improvements increase the convenient access to cryo-trapping, time-resolved X-ray crystallography empowering the MX community with efficient tools to advance research in structural biology.

PubMed: 41266557
DOI: 10.1038/s42004-025-01784-9

実験手法

X-RAY DIFFRACTION (1.3 Å)