9QXT

E. coli JetABC dimer in the DNA boarding-holding state

これはPDB形式変換不可エントリーです。

9QXT の概要

• エントリーDOI: 10.2210/pdb9qxt/pdb
• EMDBエントリー: 53444
• 分子名称: JetB, JetA, JetC, ... (4 entities in total)
• 機能のキーワード: smc complexes, wadjet, jetabcd, dna loop extrusion, dna binding protein
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 762828.33

構造登録者

Roisne-Hamelin, F.,Gruber, S. (登録日: 2025-04-16, 公開日: 2025-10-01, 最終更新日: 2025-11-19)

主引用文献

Roisne-Hamelin, F.,Liu, H.W.,Marechal, N.,Uchikawa, E.,Durand, A.,Gruber, S.
Mechanism of DNA entrapment by a loop-extruding Wadjet SMC motor.
Mol.Cell, 85:3898-, 2025

PubMed Abstract: Structural maintenance of chromosome (SMC) complexes perform critical functions by folding DNA through loop extrusion. The choreography and outcome of SMC DNA loading prior to loop extrusion, however, remain elusive. Here, we use cryo-electron microscopy to determine structures of the prokaryotic SMC Wadjet undergoing DNA loading. We show that an initial ATP-triggered relocation of both SMC dimers exposes a DNA-binding pocket and aligns two opened motor units on a DNA double helix. Subsequent ATP hydrolysis drives a nearly 360° rotation of each SMC dimer, closing the motor units around DNA in a sequential manner. This process leads to a DNA-holding conformation-an anticipated key intermediate in loop extrusion-with the DNA held within the kleisin/KITE sub-compartment. Our findings elucidate the mechanism of topological DNA loading by an SMC motor, revealing a straight DNA double helix with motor units oriented tail-to-tail in DNA-holding conformations as the likely starting point of DNA loop extrusion.

PubMed: 41072419
DOI: 10.1016/j.molcel.2025.09.015

実験手法

ELECTRON MICROSCOPY (4.13 Å)