9QHC

Crystal structure of 8-repeat CTPR protein with eliminated coordination sites

9QHC の概要

• エントリーDOI: 10.2210/pdb9qhc/pdb
• 分子名称: Engineered tetratricopeptide repeat protein, CALCIUM ION, (4S)-2-METHYL-2,4-PENTANEDIOL, ... (4 entities in total)
• 機能のキーワード: crystal engineering, protein engineering, de novo protein
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 68698.77

構造登録者

Liutkus, M.,Rojas, A.L.,Cortajarena, A.L. (登録日: 2025-03-14, 公開日: 2025-10-22)

主引用文献

Liutkus, M.,Rojas, A.L.,Cortajarena, A.L.
Rational Crystal Contact Engineering for Programmable Self-Assembled Protein Architectures.
Angew.Chem.Int.Ed.Engl., :e202516635-e202516635, 2025

PubMed Abstract: Ordered protein-based assemblies are increasingly desirable for materials science, but the design of new materials remains challenging and requires considerable effort. Crystal lattice contact modulation enables rapid rational design of an assortment of structurally diverse constructs with crystalline order. Targeted disruption of crystal contacts and directional growth pre-biasing allows for restricting crystal lattice growth in selected directions, resulting in lower-dimension assemblies with parent crystal structural features. Two-dimensional crystals, one-dimensional fibres, flexible ribbons, and single-walled nanotubes based on tetratricopeptide repeat proteins were constructed from reengineered 3D crystal lattices. The large library of available crystal structures provides an abundance of engineering targets, promising to make crystal contact engineering a rapid and attractive approach for the design of ordered supramolecular protein assemblies.

PubMed: 41077847
DOI: 10.1002/anie.202516635

実験手法

X-RAY DIFFRACTION (2.88 Å)