9QGO

Interaction between PlPVC1 baseplate and fiber in extended state

Summary for 9QGO

• Entry DOI: 10.2210/pdb9qgo/pdb
• EMDB information: 53140
• Descriptor: Tail fiber protein, Baseplate protein J-like domain-containing protein (2 entities in total)
• Functional Keywords: contractile injection system, virus like particle
• Biological source: Photorhabdus luminescens; More
• Total number of polymer chains: 6
• Total formula weight: 469952.85

Authors

Marin-Arraiza, L.,Taylor, N.M.I. (deposition date: 2025-03-14, release date: 2025-10-29, Last modification date: 2025-11-05)

Primary citation

Marin-Arraiza, L.,Roa-Eguiara, A.,Pape, T.,Sofos, N.,Hendriks, I.A.,Lund Nielsen, M.,Steiner-Rebrova, E.M.,Taylor, N.M.I.
Structural characterization of an extracellular contractile injection system from Photorhabdus luminescens in extended and contracted states.
Nat Commun, 16:9327-9327, 2025

PubMed Abstract: Contractile injection systems (CISs) are phage tail-like nanosyringes that mediate bacterial interactions by puncturing target cell membranes. Within these systems, Photorhabdus Virulence Cassettes (PVCs) can translocate toxins across eukaryotic target cell membranes and have been engineered to deliver diverse protein cargoes into non-natively-targeted organisms. Despite the structural insights into several CISs, including one PVC from P. asymbiotica, information on PVCs from other species and details on the contraction mechanism remain limited. Here, we present the single-particle cryo-electron microscopy structure of PlPVC1, a PVC from the nematode symbiont and insect pathogen Photorhabdus luminescens DJC, in both extended and contracted states. This particle displays distinct structural features that differ from other CISs, such as a cage surrounding the central spike, a larger sheath adaptor, and a plug exposed to the tube lumen. Moreover, we present the structures of the PlPVC1 fiber and the baseplate of the contracted particle, yielding insight into the contraction mechanism. This study provides structural details of the extended and contracted states of the PlPVC1 particle and supports the model in which contraction is triggered. Furthermore, it facilitates the comparison of PlPVC1 with other CISs and expands the scope of engineering opportunities for future biomedical and biotechnological applications.

PubMed: 41125615
DOI: 10.1038/s41467-025-64377-z

Experimental method

ELECTRON MICROSCOPY (6 Å)