9QGN

Cryo-EM structure of the PlPVC1 cap, 6-fold symmetrized (C6), in extended state

これはPDB形式変換不可エントリーです。

9QGN の概要

• エントリーDOI: 10.2210/pdb9qgn/pdb
• EMDBエントリー: 53139
• 分子名称: DUF4255 domain-containing protein, Phage tail protein, Phage tail sheath family protein (3 entities in total)
• 機能のキーワード: contractile injection system, virus like particle
• 由来する生物種: Photorhabdus luminescens; 詳細
• タンパク質・核酸の鎖数: 24
• 化学式量合計: 632750.69

構造登録者

Marin-Arraiza, L.,Taylor, N.M.I. (登録日: 2025-03-14, 公開日: 2025-10-29, 最終更新日: 2025-11-05)

主引用文献

Marin-Arraiza, L.,Roa-Eguiara, A.,Pape, T.,Sofos, N.,Hendriks, I.A.,Lund Nielsen, M.,Steiner-Rebrova, E.M.,Taylor, N.M.I.
Structural characterization of an extracellular contractile injection system from Photorhabdus luminescens in extended and contracted states.
Nat Commun, 16:9327-9327, 2025

PubMed Abstract: Contractile injection systems (CISs) are phage tail-like nanosyringes that mediate bacterial interactions by puncturing target cell membranes. Within these systems, Photorhabdus Virulence Cassettes (PVCs) can translocate toxins across eukaryotic target cell membranes and have been engineered to deliver diverse protein cargoes into non-natively-targeted organisms. Despite the structural insights into several CISs, including one PVC from P. asymbiotica, information on PVCs from other species and details on the contraction mechanism remain limited. Here, we present the single-particle cryo-electron microscopy structure of PlPVC1, a PVC from the nematode symbiont and insect pathogen Photorhabdus luminescens DJC, in both extended and contracted states. This particle displays distinct structural features that differ from other CISs, such as a cage surrounding the central spike, a larger sheath adaptor, and a plug exposed to the tube lumen. Moreover, we present the structures of the PlPVC1 fiber and the baseplate of the contracted particle, yielding insight into the contraction mechanism. This study provides structural details of the extended and contracted states of the PlPVC1 particle and supports the model in which contraction is triggered. Furthermore, it facilitates the comparison of PlPVC1 with other CISs and expands the scope of engineering opportunities for future biomedical and biotechnological applications.

PubMed: 41125615
DOI: 10.1038/s41467-025-64377-z

実験手法

ELECTRON MICROSCOPY (2.46 Å)