9QF5

Structure of P. furiosus 70S ribosome grown at 102deg

これはPDB形式変換不可エントリーです。

9QF5 の概要

• エントリーDOI: 10.2210/pdb9qf5/pdb
• EMDBエントリー: 53099
• 分子名称: 16S rRNA, Small ribosomal subunit protein uS8, Small ribosomal subunit protein eS8, ... (65 entities in total)
• 機能のキーワード: rna modification, cryo-em, ribosome
• 由来する生物種: Pyrococcus furiosus; 詳細
• タンパク質・核酸の鎖数: 65
• 化学式量合計: 2466834.60

構造登録者

Matzov, D.,Georgeson, J.,Westhof, E.,Schwartz, S.,Shalev-Benami, M. (登録日: 2025-03-11, 公開日: 2025-11-05, 最終更新日: 2025-12-10)

主引用文献

Garcia-Campos, M.A.,Georgeson, J.,Nir, R.,Reichelt, R.,Fluke, K.A.,Matzov, D.,Iyer, V.,Burkhart, B.W.,Lui, L.,Kustanovich, A.,Grunberger, F.,Thalalla-Gamage, S.,Howpay-Manage, S.A.,Gerovac, M.,Alexandre, N.,Nobe, Y.,Nowak, J.S.,Perera, M.,Apostle, A.,Fang, S.,Glatt, S.,Jona, G.,Ferreira-Cerca, S.,Vogel, J.,Taoka, M.,Meier, J.L.,Westhof, E.,Santangelo, T.J.,Grohmann, D.,Shalev-Benami, M.,Schwartz, S.
Pan-modification profiling facilitates a cross-evolutionary dissection of the thermoregulated ribosomal epitranscriptome.
Cell, 188:6825-, 2025

PubMed Abstract: Ribosomal RNA (rRNA) constitutes the core of ribosomes and is extensively chemically modified. Technical challenges have precluded systematically dissecting rRNA modifications and their dynamics. We develop Pan-Mod-seq, permitting inference of 16 distinct modifications across dozens of samples in parallel. We applied Pan-Mod-seq to RNA from 14 species spanning all domains of life, cultured under highly diverse conditions. While dynamic modifications are rare in mesophiles, in extreme hyperthermophiles, ∼50% of modifications are dynamic. We dissect the biogenesis and function of a conserved module of tandem mC-acC modifications, co-induced at high temperatures, via enzymes intrinsically regulated by temperature and required for growth at higher temperatures. Cryo-electron microscopy (cryo-EM) structures of ribosomes from wild-type (WT) and enzyme-deficient archaea reveal recurrent molecular interactions through which they confer structural stability, and biophysical studies demonstrate their synergistic thermostabilizing role. Our findings systematically dissect rRNA modification plasticity and pave the way for surveying the rRNA epitranscriptome in health and disease.

PubMed: 41130207
DOI: 10.1016/j.cell.2025.09.014

実験手法

ELECTRON MICROSCOPY (2.84 Å)