9QEF

Respiratory supercomplex from Mycobacterium smegmatis with decylubiquinone

9QEF の概要

• エントリーDOI: 10.2210/pdb9qef/pdb
• EMDBエントリー: 53065
• 分子名称: Cytochrome bc1 complex cytochrome c subunit, Uncharacterized protein MSMEG_4692/MSMEI_4575, LpqE protein, ... (33 entities in total)
• 機能のキーワード: respiratory supercomplex, membrane protein, actinobacteria, electron transport
• 由来する生物種: Mycolicibacterium smegmatis; 詳細
• タンパク質・核酸の鎖数: 28
• 化学式量合計: 808260.93

構造登録者

Kovalova, T.,Krol, S.,Brzezinski, P.,Hogbom, M. (登録日: 2025-03-09, 公開日: 2026-01-21)

主引用文献

Krol, S.,Kovalova, T.,Janczak, M.,Kalsum, S.,Akber, M.,Hogbom, M.,Brighenti, S.,Adelroth, P.,Brzezinski, P.
Mycobacterial respiratory chain enzymes and growth are inhibited by decylubiquinone.
Commun Biol, 9:43-43, 2025

PubMed Abstract: Aerobic organisms obtain energy by linking electron transfer from NADH to O, through the respiratory chain, to transmembrane proton translocation. In mycobacteria the respiratory chain is branched; the membrane-bound electron carrier menaquinol (MQH) donates electrons either to the O-reducing cytochrome bd or a supercomplex that is composed of a complex (C) III dimer flanked by two CIVs. Here, we measured the dimethyl-naphthoquinone (DMNQH a menaquinol analogue) oxidation:O reduction activities of the CIIICIV supercomplex and cytochrome bd in the presence of an analogue (decylubiquinone, DCQ) of the mammalian electron carrier, ubiquinol. The data show that DCQH inhibits both the CIIICIV and cytochrome bd activities, suggesting that DCQ/DCQH interferes with both branches of the respiratory chain. Cryo-EM data of the M. smegmatis supercomplex shows that oxidized DCQ binds in the electron donor site (Q) of CIII. Accordingly, growth of M. smegmatis cells was impaired in the presence of DCQ. Remarkably, DCQ also impairs intracellular growth of virulent M. tuberculosis cells in human primary macrophages suggesting that the compound could potentially be used as an adjuvant during tuberculosis disease treatment.

PubMed: 41372535
DOI: 10.1038/s42003-025-09309-9

実験手法

ELECTRON MICROSCOPY (2.5 Å)