9QED

Cryo-EM structure of the XPF-ERCC1-SLX4(330-555)-SLX4IP complex

9QED の概要

• エントリーDOI: 10.2210/pdb9qed/pdb
• EMDBエントリー: 53055
• 分子名称: Protein SLX4IP, DNA repair endonuclease XPF, DNA excision repair protein ERCC-1, ... (4 entities in total)
• 機能のキーワード: dna repair, endonuclease, multiprotein complex, hydrolase
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 214738.19

構造登録者

Feng, J.,Cronin, N.B.,Greber, B.J. (登録日: 2025-03-09, 公開日: 2025-12-17, 最終更新日: 2026-01-28)

主引用文献

Feng, J.,Martin, P.R.,Kowalski, S.,Lecot, M.,Cronin, N.B.,Matthews-Palmer, T.,Niedzwiedz, W.,Greber, B.J.
Molecular basis of XPF-ERCC1 targeting to SLX4-dependent DNA repair pathways.
Nat Commun, 17:522-522, 2025

PubMed Abstract: The preservation and faithful propagation of genetic information is essential for all life forms and depends on cellular pathways that enable replication, recombination, and repair of DNA. The multifunctional XPF-ERCC1 DNA endonuclease complex acts in several DNA repair pathways and interacts with numerous partner proteins and large DNA repair assemblies, including the nucleotide excision repair machinery and the SMX tri-endonuclease complex. Here, we report structures of XPF-ERCC1 in complex with the DNA repair factors SLX4 and SLX4IP, thereby identifying key residues responsible for direct interactions with XPF-ERCC1. When introduced into human cells, point mutations in these interfaces impair the interactions between XPF-ERCC1 and SLX4 or SLX4IP, and disruption of the XPF-SLX4IP interface leads to cis-platin sensitivity. Furthermore, our data reveal the structure of the human XPF-ERCC1-SLX4IP-SLX4 complex with DNA bound at its active site, and they complete the structural characterisation of molecular interactions required to assemble the SMX complex.

PubMed: 41402316
DOI: 10.1038/s41467-025-67216-3

実験手法

ELECTRON MICROSCOPY (3.2 Å)