9QCE

HA70-HA17-HA33 complex from Clostridium botulinum Serotype B1

これはPDB形式変換不可エントリーです。

9QCE の概要

• エントリーDOI: 10.2210/pdb9qce/pdb
• EMDBエントリー: 53010
• 分子名称: Hemagglutinin component HA70, Hemagglutinin component HA17, Hemagglutinin component HA33 (3 entities in total)
• 機能のキーワード: progenitor-toxin complex, botulinum neurotoxin, hemagglutinin, toxin
• 由来する生物種: Clostridium botulinum; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 298042.15

構造登録者

Krc, A.,Persson Kosenina, S.,Masuyer, G.,Stenmark, P. (登録日: 2025-03-04, 公開日: 2025-09-10)

主引用文献

Krc, A.,Kosenina, S.P.,Nowakowska, M.B.,Masuyer, G.,Stenmark, P.
Structure of the complete 14-subunit botulinum neurotoxin B complex reveals a unique anchoring through the narrow central pore of HA70.
Sci Adv, 11:eadx5058-eadx5058, 2025

PubMed Abstract: Botulinum neurotoxin serotype B1 (BoNT/B) is a highly potent neurotoxin and therapeutic agent. Here, we present the structure of the complete 14-subunit (780 kDa) progenitor toxin complex (L-PTC) and of five subcomplexes. The structures show how the toxin interacts with its associated components in their role to protect and deliver BoNT/B across epithelial barriers. Each subcomplex, including the M-PTC, M-PTC-HA70, NTNH-HA70, and HA70 trimer, provides detailed understanding of the assembly mechanism, in which the NTNH-nLoop adopts a unique fold that locks the M-PTC into a central pore formed by HA70. The HA subcomplex presents a tripod architecture with flexible legs that may adapt to the rugged cell surface. Mass photometry reveals the pH dependence of BoNT/B release from the complex which is unexpectedly influenced by the presence of HA70. This study provides the complete L-PTC structure, offering insights into its assemblage and supporting the development of countermeasures and therapeutic applications.

PubMed: 40864696
DOI: 10.1126/sciadv.adx5058

実験手法

ELECTRON MICROSCOPY (2.8 Å)