9QAJ

Structure of the nucleosome-bound human BCL7A

9QAJ の概要

• エントリーDOI: 10.2210/pdb9qaj/pdb
• EMDBエントリー: 52975
• 分子名称: Histone H3.2, Histone H4, Histone H2A, ... (8 entities in total)
• 機能のキーワード: nucleosome, acidic patch, bcl7a, arginine anchor, structural protein
• 由来する生物種: Xenopus laevis (African clawed frog); 詳細
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 203172.98

構造登録者

Martin, F.,Bergamin, E. (登録日: 2025-02-28, 公開日: 2025-04-23)

主引用文献

Martin, F.,Kazrani, A.A.,Lafouge, J.,Diaz-Jimenez, D.M.,Siebert, S.,Fabbro-Burtschell, L.,Maillard, E.,Lapouge, K.,Mertens, H.D.T.,Sauter, C.,Leitner, A.,Ochsenbein, F.,Blais, A.,Bergamin, E.
Structure of the nucleosome-bound human BCL7A.
Nucleic Acids Res., 53:-, 2025

PubMed Abstract: Proteins of the BCL7 family (BCL7A, BCL7B, and BCL7C) are among the most recently identified subunits of the mammalian SWI/SNF chromatin remodeler complex and are absent from the unicellular version of this complex. Their function in the complex is unknown, and very limited structural information is available, despite the fact that they are mutated in several cancer types, most notably blood malignancies and hence medically relevant. Here, using cryo-electron microscopy in combination with biophysical and biochemical approaches, we show that BCL7A forms a stable, high-affinity complex with the nucleosome core particle (NCP) through binding of BCL7A with the acidic patch of the nucleosome via an arginine anchor motif. This interaction is impaired by BCL7A mutations found in cancer. Further, we determined that BCL7A contributes to the remodeling activity of the mSWI/SNF complex and we examined its function at the genomic level. Our findings reveal how BCL7 proteins interact with the NCP and help rationalize the impact of cancer-associated mutations. By providing structural information on the positioning of BCL7 on the NCP, our results broaden the understanding of the mechanism by which SWI/SNF recognizes the chromatin fiber.

PubMed: 40207634
DOI: 10.1093/nar/gkaf273

実験手法

ELECTRON MICROSCOPY (2.95 Å)