9PSD

High-resolution crystal structure of Vibrio cholerae NFeoB bound to GDP-AlF3

9PSD の概要

• エントリーDOI: 10.2210/pdb9psd/pdb
• 分子名称: Ferrous iron transport protein B, GLYCEROL, GUANOSINE-5'-DIPHOSPHATE, ... (6 entities in total)
• 機能のキーワード: feo, iron, membrane protein, transporter, gtpase, ntpase, metal transport
• 由来する生物種: Vibrio cholerae
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 119580.03

構造登録者

Magante, K.,Lee, M.,Smith, A.T. (登録日: 2025-07-25, 公開日: 2026-06-03)

主引用文献

Magante, K.,Armstrong, C.M.,Lee, M.,Smith, A.T.
The Structure of the Full Catalytic Cycle of Vibrio cholerae NFeoB.
J.Mol.Biol., 438:169727-169727, 2026

PubMed Abstract: The acquisition of iron is critical for the survival and the virulence of numerous infectious pathogens, and most bacteria acquire ferrous iron (Fe) by utilizing the ferrous iron transport (Feo) system. FeoB is the main component of this system and its function is regulated by its soluble cytosolic domain, termed NFeoB. We have recently begun to define the structure and the mechanism of the Feo system from the bacterium Vibrio cholerae, the causative agent of the disease cholera. However, major structural gaps in our understanding of the nucleotide-promiscuous V. cholerae NFeoB still exist. In this work, we have determined several new X-ray crystal structures that reveal distinct snapshots of the VcNFeoB domain in uncommon and unprecedented states, ultimately illuminating the full catalytic cycle of this NTPase. This work reveals important functional features of VcNFeoB that may be leveraged and ultimately targeted to prevent the infectivity and the spread of cholera.

PubMed: 41724262
DOI: 10.1016/j.jmb.2026.169727

実験手法

X-RAY DIFFRACTION (2 Å)