9PSC

Crystal structure of C. elegans PUF-3 in complex with pos-1 RNA mut1

9PSC の概要

• エントリーDOI: 10.2210/pdb9psc/pdb
• 分子名称: PUM-HD domain-containing protein, pos-1 RNA mut1, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: rna binding, caenorhabditis elegans, puf protein, rna binding protein
• 由来する生物種: Caenorhabditis elegans; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 50791.78

構造登録者

Zhang, Y.,Hall, T.M.T. (登録日: 2025-07-25, 公開日: 2025-12-17, 最終更新日: 2026-01-28)

主引用文献

Zhang, Y.,Kennedy, F.D.,Hall, T.M.T.
Crystal structures of Caenorhabditis elegans PUF-3 depict plasticity of RNA recognition that enables germline gene regulation.
Nucleic Acids Res., 54:-, 2026

PubMed Abstract: Prototypical PUF proteins are known for modular, sequence-specific RNA recognition. A single PUF protein in Drosophila melanogaster Pumilio and two closely related proteins in mammals, PUM1 and PUM2, are representative. In contrast, Caenorhabditis elegans PUF proteins have evolved to include four subfamilies of single-stranded RNA-binding proteins that recognize distinct sequence elements. Here, we provide an in-depth structural and biochemical analysis of C. elegans PUF-3, a member of the subfamily lacking structural information. We determined crystal structures of PUF-3 in complex with RNAs representing three classes of binding elements. These structures and quantitative RNA-binding assays fine-tune the features of PUF-3 RNA recognition and demonstrate how PUF-3 binds to varied target RNA elements by accommodating divergent sequences between conserved features. Our analyses here and published biological data suggest that PUF-3 may redundantly regulate RNA targets with other PUF proteins. We determined the in vitro binding affinities of C. elegans PUF protein subfamily representatives for the range of corresponding RNA elements. We find that PUF-3 and FBF-2 have broader RNA recognition, whereas PUF-8 and PUF-6 are highly selective. These subfamily recognition properties and protein expression patterns support a working model for PUF protein activities in different regions of the C. elegans germline.

PubMed: 41495898
DOI: 10.1093/nar/gkaf1431

実験手法

X-RAY DIFFRACTION (2.4 Å)