9P3L

Structure of ANDV dimer of tetramer at conformation III

これはPDB形式変換不可エントリーです。

9P3L の概要

• エントリーDOI: 10.2210/pdb9p3l/pdb
• EMDBエントリー: 71242
• 分子名称: Glycoprotein N, Glycoprotein C, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: andv, hantavirus glycoprotein, dimer of tetramer, gn and gc, viral protein
• 由来する生物種: Orthohantavirus andesense; 詳細
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 1070711.02

構造登録者

Luqiang, G.,McLellan, J.S. (登録日: 2025-06-14, 公開日: 2025-07-30, 最終更新日: 2026-03-25)

主引用文献

Guo, L.,McFadden, E.,Slough, M.M.,Stone, E.T.,Berrigan, J.,Mittler, E.,Hatzakis, K.,Hinkley, T.,Kain, H.S.,Ke, Z.,Warner, N.L.,Erasmus, J.H.,Chandran, K.,McLellan, J.S.
High-resolution in situ structures of hantavirus glycoprotein tetramers.
Cell, 2026

PubMed Abstract: New World hantaviruses cause severe infections in humans. Previous structural studies have advanced our understanding of hantavirus glycoprotein architecture; however, the lack of high-resolution structures of the glycoprotein tetramer and its lattice organization has limited mechanistic insights into viral assembly and entry. Here, we leveraged a virus-like particle (VLP) system to establish a cryo-electron microscopy workflow for lattice-forming viral glycoproteins. This enabled the determination of a 2.35 Å resolution structure of the membrane-embedded Andes virus (ANDV) glycoprotein tetramer as well as the structures of dimers of tetramers and a complex with antibody ADI-65534. These structures reveal previously uncharacterized features of glycoprotein organization, stability, and pH sensing. The immunization of mice with self-amplifying replicon RNA (repRNA) encoding ANDV-VLPs elicited high levels of glycoprotein-binding antibodies but equivalent titers of neutralizing antibodies compared with the repRNA-encoded native ANDV glycoprotein complex. These findings advance our understanding of hantavirus glycoprotein assemblies, laying a foundation for structure-based vaccine design.

PubMed: 41763200
DOI: 10.1016/j.cell.2026.01.030

実験手法

ELECTRON MICROSCOPY (3.37 Å)