9OZY
Gradient equilibration of hexagonal thermolysin to low salt over 15 minutes
Summary for 9OZY
| Entry DOI | 10.2210/pdb9ozy/pdb |
| Descriptor | Thermolysin, VALINE, LYSINE, ... (7 entities in total) |
| Functional Keywords | glycosidase, hydrolase |
| Biological source | Geobacillus stearothermophilus |
| Total number of polymer chains | 1 |
| Total formula weight | 34930.50 |
| Authors | Juers, D.H. (deposition date: 2025-06-06, release date: 2025-10-15, Last modification date: 2025-11-12) |
| Primary citation | Juers, D.H.,Quire, J.,Stothers, S. Automated gradient equilibration of macromolecular crystals to new solution conditions. Acta Crystallogr.,Sect.F, 81:478-486, 2025 Cited by PubMed Abstract: We describe a device and a method for changing the ambient solution of a macromolecular crystal. The approach is gentle, automated, inexpensive and open source. Examples are given of the equilibration of three different crystals to new solutions with exchange times ranging from 5 to 180 min. In each case direct transfer of the crystal to the new solution causes cracking, which is eliminated with gradient equilibration using the described device. Crystals equilibrated with the device produce high-quality diffraction that yields refined structures comparable to those determined previously. The device offers a more systematic and labor-saving workflow than current practice both for performing diffraction analysis of macromolecular crystals and for investigating the response of macromolecular crystals to changes in solution composition. PubMed: 41042616DOI: 10.1107/S2053230X25008398 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.75 Å) |
Structure validation
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