9OQ8

Crystal structure of selenomethionine-substituted cyclodehydratase RohQ

9OQ8 の概要

• エントリーDOI: 10.2210/pdb9oq8/pdb
• 関連するPDBエントリー: 9c5w
• 分子名称: RohQ, IMIDAZOLE (3 entities in total)
• 機能のキーワード: azomycin biosynthetic protein, duf6190, cyclodehydratase, de novo emerged enzyme, biosynthetic protein
• 由来する生物種: Pseudomonas brassicacearum
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 112342.74

構造登録者

Wei, Z.-W.,Daniel-Ivad, P.,Ryan, K.S. (登録日: 2025-05-20, 公開日: 2025-07-23)

主引用文献

Wei, Z.W.,Daniel-Ivad, P.,Zhang, L.,Ryan, K.S.
Structure and Mechanism of the Azomycin Biosynthetic Enzyme RohQ That Catalyzes a Spontaneous Cyclodehydration.
J.Am.Chem.Soc., 2025

PubMed Abstract: RohQ from the azomycin biosynthetic pathway catalyzes a spontaneous cyclodehydration to form 2-aminoimidazole. Here we report the structure and mechanism of RohQ and use a serendipitously bound imidazole to pinpoint active site residues. We propose that catalysis occurs at the dimeric interface using two key aspartic acid residues for proton transfer steps to accelerate 3 × 10-fold intramolecular cyclization of a guanidino group and aldehyde, releasing water. Our work expands our understanding of emerged enzymes and provides the first structural and mechanistic view of a yet-unexplored protein family.

PubMed: 40644315
DOI: 10.1021/jacs.5c04341

実験手法

X-RAY DIFFRACTION (2.38 Å)