9OLF

Membrane-associated human mitoribosome in complex with TACO1

これはPDB形式変換不可エントリーです。

9OLF の概要

• エントリーDOI: 10.2210/pdb9olf/pdb
• EMDBエントリー: 70592
• 分子名称: 39S ribosomal protein L32, mitochondrial, 39S ribosomal protein L41, mitochondrial, SPERMINE, ... (105 entities in total)
• 機能のキーワード: mitochondria, mitoribosome, inner membrane, ribosome
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 100
• 化学式量合計: 3417154.03

構造登録者

Wang, S.,Xiong, Y.,Zhang, Y. (登録日: 2025-05-12, 公開日: 2026-02-18)

主引用文献

Wang, S.,Brischigliaro, M.,Zhang, Y.,Wu, C.,Zheng, W.,Barrientos, A.,Xiong, Y.
Structural basis of TACO1-mediated efficient mitochondrial translation.
Nat Commun, 2026

PubMed Abstract: Translation elongation is a universally conserved step in protein synthesis, relying on elongation factors that engage the ribosomal L7/L12 stalk to mediate aminoacyl-tRNA delivery, accommodation, and ribosomal translocation. Using in organello cryo-electron microscopy, we reveal how the mitochondrial translation accelerator TACO1 promotes efficient elongation on human mitoribosomes. TACO1 binds the mitoribosomal region typically bound by elongation factor Tu (mtEF-Tu), bridging the large and small subunits via contacts with 16S rRNA, bL12m, A-site tRNA, and uS12m. While active throughout elongation, TACO1 is especially critical when translating polyproline motifs. Its absence prolongs mtEF-Tu residence in A/T states, causes persistent mitoribosomal stalling and premature subunit dissociation. Structural analyses indicate that TACO1 competes with mtEF-Tu for mitoribosome binding, stabilizes A-site tRNA, and enhances peptidyl transfer through a mechanism distinct from EF-P and eIF5A. These findings suggest that bacterial TACO1 orthologs may serve analogous roles, highlighting an evolutionarily conserved strategy for maintaining elongation efficiency during challenging translation events.

PubMed: 41663403
DOI: 10.1038/s41467-026-69156-y

実験手法

ELECTRON MICROSCOPY (2.46 Å)