9OKE

Structure of the Bombyx mori bmCENP-HIKM-LN-T-OP complex without the CS module

9OKE の概要

• エントリーDOI: 10.2210/pdb9oke/pdb
• 関連するPDBエントリー: 9OKB 9OKD
• EMDBエントリー: 70561
• 分子名称: bmCENP-H, bmCENP-K, Centromere protein I, ... (9 entities in total)
• 機能のキーワード: ccan, complex, segregation, dna binding protein
• 由来する生物種: Bombyx mori (domestic silkworm); 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 445232.41

構造登録者

Yatskevich, S.,Ciferri, C. (登録日: 2025-05-09, 公開日: 2026-07-08)

主引用文献

Yu, C.,Sankaranarayanan, S.R.,Cornilleau, G.,Howes, A.C.,Azumaya, C.M.,Day, E.S.,Zilberleyb, I.,Brillantes, B.,Cheung, T.K.,Dec, L.,Loew, D.,Tran, P.,Rose, C.M.,Drinnenberg, I.A.,Ciferri, C.,Yatskevich, S.
Architecture and function of holocentric CENP-A-independent inner kinetochores.
Sci Adv, 12:eaeb5817-eaeb5817, 2026

PubMed Abstract: Kinetochores are essential macromolecular complexes anchoring chromosomes to the mitotic spindle, ensuring faithful cell division. Despite their critical role, the structural organization of kinetochores across diverse species remains poorly understood. We present the inner kinetochore constitutive centromere-associated network (CCAN) structure of the silkmoth , an insect that lacks the canonical centromere-specifying histone variant CENP-A and exhibits chromosome-wide centromeric activity (holocentric). The CCAN incorporates four previously uncharacterized centromeric subunit proteins that are structurally related to the Dam1/DASH complex but function in scaffolding the inner kinetochore rather than in microtubule binding. Similar to the yeast and human systems, the CCAN also entraps DNA within its central closed chamber. However, unlike these systems, the CCAN can also assemble in vitro into a self-contained head-to-head dimer via atypical histone-fold protein dimerization. On the basis of our findings, we propose that the holocentric organization may emerge from the modular arrangement of discrete kinetochore units.

PubMed: 42319948
DOI: 10.1126/sciadv.aeb5817

実験手法

ELECTRON MICROSCOPY (3.24 Å)