9OEE

S. griseus TUA bound UmbA4 complexes

これはPDB形式変換不可エントリーです。

9OEE の概要

• エントリーDOI: 10.2210/pdb9oee/pdb
• EMDBエントリー: 70396
• 分子名称: Secreted esterase, 2-acetamido-2-deoxy-alpha-D-galactopyranose, 2,3-diacetamido-2,3-dideoxy-beta-D-mannopyranuronic acid, ... (4 entities in total)
• 機能のキーワード: teichuronic acid, umba4, umbrella toxin, seattle structural genomics center for infectious disease, ssgcid, toxin
• 由来する生物種: Streptomyces coelicolor
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 381172.78

構造登録者

Park, Y.J.,Zhao, Q.,Seattle Structural Genomics Center for Infectious Disease (SSGCID),DiMaio, F.,Mougous, J.D.,Veesler, D. (登録日: 2025-04-28, 公開日: 2025-12-17)

主引用文献

Zhao, Q.,Vlach, J.,Park, Y.J.,Tan, Y.,Bertolli, S.K.,Srinivas, P.,Liao, P.,Fitzpatrick, C.R.,Dangl, J.L.,Azadi, P.,DiMaio, F.,Peterson, S.B.,Zhang, D.,Veesler, D.,Mougous, J.D.
The unique architecture of umbrella toxins permits a two-tiered molecular bet-hedging strategy for interbacterial antagonism.
Cell, 2025

PubMed Abstract: Bacteria exist in competitive and rapidly changing environments in which the nature of future threats cannot be easily predicted. Streptomyces coelicolor produces three antibacterial umbrella particles that harbor distinct polymorphic toxin domains and an overlapping set of six diversified lectins. Here, we show that the exquisite specificity of umbrella particles derives from lectin-mediated species-specific binding to previously undescribed hypervariable surface glycoconjugates. A cryo-electron microscopy (cryo-EM) structure of one such lectin in complex with its oligosaccharide substrate defines the molecular basis for targeting through the coordinated recognition of multiple glycan features. Biochemical and genetic studies of several target species, in conjunction with lectin-swapping experiments, support a model whereby S. coelicolor umbrella toxin diversification at the levels of lectin composition and toxin polymorphism represents a unique, two-tiered bet-hedging strategy. Bioinformatic analyses support this as a means by which the unusual architecture of umbrella toxins offers Streptomyces a generalizable strategy to antagonize an unpredictable array of competitors.

PubMed: 41338195
DOI: 10.1016/j.cell.2025.10.044

実験手法

ELECTRON MICROSCOPY (3.4 Å)