9OAD

C1 reconstruction of the thermophilic bacteriophage P74-26 Portal and Portal Vertex

これはPDB形式変換不可エントリーです。

9OAD の概要

• エントリーDOI: 10.2210/pdb9oad/pdb
• EMDBエントリー: 70280
• 分子名称: Major head protein, P74-26 Head Decoration Protein, Portal Vertex Protein, ... (7 entities in total)
• 機能のキーワード: bacteriophage, thermophilic, neck, portal, virus
• 由来する生物種: Oshimavirus P7426; 詳細
• タンパク質・核酸の鎖数: 79
• 化学式量合計: 2170004.95

構造登録者

Sedivy, E.L.,Agnello, E.,Song, K.,Xu, C.,Kelch, B.A. (登録日: 2025-04-21, 公開日: 2026-01-28)

主引用文献

Sedivy, E.L.,Agnello, E.,Hobaugh, J.E.,Ahsan, R.,Song, K.,Xu, C.,Kelch, B.A.
The structure of a thermostable phage's portal vertex and neck complex illuminates the headful maturation mechanism.
J.Mol.Biol., :169641-169641, 2026

PubMed Abstract: Viruses assemble from component parts inside their host cells, but the mechanisms coordinating this complex process are not completely understood. In tailed bacteriophages, the genome is packaged into its capsid shell through the portal complex. The portal complex then closes to retain DNA and connects to the tail, which is required for host recognition and infection. The trigger to stop pumping DNA and assemble the mature virus has been a longstanding conundrum in the field. We determined the structure of the portal, the proteins that connect it to the tail, and portal vertex in the hyperthermophilic phage Oshimavirus using cryo-Electron Microscopy (cryo-EM). We find highly intertwined loop structures, like in a wicker basket, potentially stabilizing the portal vertex against high temperatures. Moreover, we observe that the portal protrudes from the capsid in mature virions. We propose that portal is repositioned by packaged DNA, forming a pressure-sensitive switch that terminates genome packaging and triggers tail attachment in headful phages.

PubMed: 41544933
DOI: 10.1016/j.jmb.2026.169641

実験手法

ELECTRON MICROSCOPY (3.56 Å)