9O7M

Cryo-EM Structure of YfdQ Reveals a Widespread Novel Family of Bacteriophage-Associated Proteins with Shell-Like Assemblies

9O7M の概要

• エントリーDOI: 10.2210/pdb9o7m/pdb
• EMDBエントリー: 70204
• 分子名称: YfdQ Protein (1 entity in total)
• 機能のキーワード: d18 symmetry, shell-like structure, duf2303, yfdq, viral protein
• 由来する生物種: Caudoviricetes
• タンパク質・核酸の鎖数: 36
• 化学式量合計: 1060456.14

構造登録者

Guzzo, C.R.,Araujo, G.G.,Merighi, D.G.S. (登録日: 2025-04-15, 公開日: 2026-07-08)

主引用文献

Merighi, D.G.S.,Pompeu, G.C.,Araujo, G.G.,Silva de Souza, A.,Sgro, G.G.,Farah, C.S.,Guzzo, C.R.
Cryo-EM structure of YfdQ reveals a widespread family of bacteriophage-associated proteins with shell-like assemblies.
Structure, 2026

PubMed Abstract: Bacteriophages are key players in microbial ecosystems with strong biotechnological potential, yet many phage-encoded proteins remain uncharacterized. We present the structure of the YfdQ complex from the Xanthomonas citri ΦXacm4-11 phage, a conserved DUF2303 protein encoded by double-stranded DNA bacteriophages of the Caudoviricetes class. Using cryo-electron microscopy, we report that native YfdQ assembles into a shell-like structure with D18 symmetry forming a ∼1.1 MegaDalton complex, a hollow interior and a unique equatorial β strand stitching oligomerization interface that holds together the two hemispheres. YfdQ homologs are widely distributed among phages infecting diverse bacterial hosts, suggesting a conserved biological role. YfdQ are found in prophages and cryptic phages. Our results suggest that heterologous expression of YfdQ in X. citri and in Escherichia coli allows the obtention of structurally heterogeneous YfdQ complexes, suggesting that its proper assembly into the shell-like structure may depend on still unidentified viral factors.

PubMed: 42309054
DOI: 10.1016/j.str.2026.05.009

実験手法

ELECTRON MICROSCOPY (3.9 Å)