9NR6

The structure of Noelin 1 with cerebellar GluA1/A4-ATD

これはPDB形式変換不可エントリーです。

9NR6 の概要

• エントリーDOI: 10.2210/pdb9nr6/pdb
• EMDBエントリー: 49723
• 分子名称: Glutamate receptor 1, Glutamate receptor 4, 11B8 scFv, ... (8 entities in total)
• 機能のキーワード: iglur, cp-ampa receptors, noelin 1, membrane protein
• 由来する生物種: Mus musculus; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 298130.43

構造登録者

Fang, C.L.,Gouaux, E. (登録日: 2025-03-14, 公開日: 2025-07-02, 最終更新日: 2025-11-26)

主引用文献

Fang, C.,Spangler, C.J.,Park, J.,Sheldon, N.,Trussell, L.O.,Gouaux, E.
Gating and noelin clustering of native Ca 2+ -permeable AMPA receptors.
Nature, 645:526-534, 2025

PubMed Abstract: AMPA-type ionotropic glutamate receptors (AMPARs) are integral to fast excitatory synaptic transmission and have vital roles in synaptic plasticity, motor coordination, learning and memory. Whereas extensive structural studies have been conducted on recombinant AMPARs and native calcium-impermeable (CI)-AMPARs alongside their auxiliary proteins, the molecular architecture of native calcium-permeable (CP)-AMPARs has remained undefined. Here, to determine the subunit composition, physiological architecture and gating mechanisms of CP-AMPARs, we visualize these receptors, immunoaffinity purified from rat cerebella, and resolve their structures using cryo-electron microscopy (cryo-EM). Our results indicate that the predominant assembly consists of GluA1 and GluA4 subunits, with the GluA4 subunit occupying the B and D positions, and auxiliary subunits, including transmembrane AMPAR regulatory proteins (TARPs) located at the B' and D' positions, and cornichon homologues (CNIHs) or TARPs located at the A' and C' positions. Furthermore, we resolved the structure of the noelin (NOE1)-GluA1-GluA4 complex, in which NOE1 specifically binds to the GluA4 subunit at the B and D positions. Notably, NOE1 stabilizes the amino-terminal domain layer without affecting gating properties of the receptor. NOE1 contributes to AMPAR function by forming dimeric AMPAR assemblies that are likely to engage in extracellular networks, clustering receptors in synaptic environments and modulating receptor responsiveness to synaptic inputs.

PubMed: 40550474
DOI: 10.1038/s41586-025-09289-0

実験手法

ELECTRON MICROSCOPY (3.26 Å)