9NQD

Structure of Pseudomonas FapC Biofilm-Forming Functional Amyloid

これはPDB形式変換不可エントリーです。

9NQD の概要

• エントリーDOI: 10.2210/pdb9nqd/pdb
• EMDBエントリー: 49649
• 分子名称: Functional amyloid subunit FapC (1 entity in total)
• 機能のキーワード: fapc functional amyloid pseudomonas c, amyloid, fibril, biofilm, functional amyloid, structural protein
• 由来する生物種: Pseudomonas fluorescens
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 270890.81

構造登録者

Hansen, K.H.,Golcuk, M.,Byeon, C.B.,Plechinger, E.B.,Conway, J.F.,Andreasen, M.,Gur, M.,Akbey, U. (登録日: 2025-03-12, 公開日: 2025-06-25, 最終更新日: 2025-10-08)

主引用文献

Hansen, K.H.,Golcuk, M.,Byeon, C.H.,Tunc, A.,Plechinger, E.B.,Dueholm, M.K.D.,Conway, J.F.,Andreasen, M.,Gur, M.,Akbey, U.
Structural basis of Pseudomonas biofilm-forming functional amyloid FapC formation.
Sci Adv, 11:eadx7829-eadx7829, 2025

PubMed Abstract: Biofilm-protected causes chronic infections that are difficult to treat. FapC, the major biofilm-forming functional amyloid in , is essential for biofilm integrity, yet its structural details remain unresolved. Using an integrative structural biology approach, we combine a solution nuclear magnetic resonance-based structural ensemble of unfolded monomeric FapC, a ~3.3-angstrom-resolution cryo-electron microscopy (cryo-EM) density map of FapC fibril, and all-atom molecular dynamics (MD) simulations to capture the transition from the unfolded to folded monomer to the fibrillar fold, providing a complete structural view of FapC biogenesis. Cryo-EM reveals a unique irregular triple-layer β solenoid cross-β fibril composed of a single protofilament. MD simulations initiated from monomeric and fibrillar FapC mapped structural transitions, offering mechanistic insights into amyloid assembly and disassembly. Understanding FapC reveals how exploits functional amyloids for biofilm formation, and establishes a structural and mechanistic foundation for developing therapeutics targeting biofilm-related infection and antimicrobial resistance.

PubMed: 40991694
DOI: 10.1126/sciadv.adx7829

実験手法

ELECTRON MICROSCOPY (3.3 Å)