9NGO

CRYO-EM STRUCTURE OF HUMAN U7 SNRNP WITH METHYLATED noncleavable H2A* SUBSTRATE PRE-MRNA (FOCUS MAP)

9NGO の概要

• エントリーDOI: 10.2210/pdb9ngo/pdb
• 関連するPDBエントリー: 9N96 9NB1
• EMDBエントリー: 49389 49403
• 分子名称: Cleavage and polyadenylation specificity factor subunit 3, Cleavage and polyadenylation specificity factor subunit 2, Symplekin (3 entities in total)
• 機能のキーワード: methylated rna, 3' end processing, u7 snrnp, histone pre-mrna, rna binding protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 286533.74

構造登録者

Desotell, A.,Tong, L. (登録日: 2025-02-22, 公開日: 2026-01-21)

主引用文献

Desotell, A.,Marzluff, W.F.,Dominski, Z.,Tong, L.
An N-terminal helix of Lsm11 stabilizes CPSF73 in U7 snRNP for histone pre-mRNA 3'-end processing.
Nucleic Acids Res., 54:-, 2026

PubMed Abstract: The U7 snRNP (small nuclear ribonucleoprotein) is responsible for the 3'-end processing of replication-dependent histone messenger RNA precursors (pre-mRNAs). A helix in the Lsm11 N-terminal extension contacts the metallo-β-lactamase domain of the U7 snRNP endonuclease CPSF73. We mutated or deleted this helix and found that the mutant machineries had substantially reduced cleavage activity toward the pre-mRNA. Our cryo-electron microscopy (cryo-EM) studies indicated that the helix was important for helping to hold CPSF73 in its correct position for the cleavage reaction. We also reconstituted a wild-type U7 snRNP in complex with a methylated, noncleavable pre-mRNA. We observed that CPSF73 could achieve an open conformation independent of RNA binding to its active site. Finally, we found that a previously uninterpreted EM density for a small helix at the CPSF73-CPSF100 interface belonged to the C-terminal end of CstF77, copurified from insect cells and highly conserved among CstF77 homologs. This CstF77 binding site had a small effect on the cleavage activity of U7 snRNP. Overall, our studies have revealed the importance of the conserved helix in the Lsm11 N-terminal extension for U7 snRNP, provided structural evidence that CPSF73 can achieve an open, active conformation without RNA binding in its active site, and identified a previously unknown binding site for CstF77 in CPSF100.

PubMed: 41495886
DOI: 10.1093/nar/gkaf1442

実験手法

ELECTRON MICROSCOPY (3.01 Å)