9NGF

ELIC facing ECD outwards in liposomes with 2:1:1 POPC:POPE:POPG

9NGF の概要

• エントリーDOI: 10.2210/pdb9ngf/pdb
• EMDBエントリー: 49383
• 分子名称: Erwinia chrysanthemi ligand-gated ion channel, SODIUM ION (2 entities in total)
• 機能のキーワード: elic, ion channel, plgic, structural protein, membrane protein, transport protein
• 由来する生物種: Dickeya dadantii
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 184509.95

構造登録者

Dalal, V.,Cheng, W.W.L. (登録日: 2025-02-22, 公開日: 2025-07-23, 最終更新日: 2025-09-17)

主引用文献

Dalal, V.,Tan, B.K.,Xu, H.,Cheng, W.W.L.
Cryo-EM structures of a pentameric ligand-gated ion channel in liposomes.
Elife, 14:-, 2025

PubMed Abstract: Detergents and lipid nanodiscs affect the cryo-EM structures of pentameric ligand-gated ion channels (pLGICs) including ELIC. To determine the structure of a pLGIC in a membrane environment that supports ion channel function, we performed single particle cryo-EM of ELIC in liposomes. ELIC activation and desensitization were confirmed in liposomes with a stopped-flow thallium flux assay. Using WT ELIC and a non-desensitizing mutant (ELIC5), we captured resting, activated, and desensitized structures at high resolution. In the desensitized structure, the ion conduction pore has a constriction at the 9' leucine of the pore-lining M2 helix, indicating that 9' is the desensitization gate in ELIC. The agonist-bound structures of ELIC in liposomes are distinct from those in nanodiscs. In general, the transmembrane domain is more loosely packed in liposomes compared to nanodiscs. It has been suggested that large nanodiscs are superior for supporting membrane protein function. However, ELIC localizes to the rim of large circularized nanodiscs, and structures of ELIC in large nanodiscs deviate from the liposome structures more than those in small nanodiscs. Using liposomes for cryo-EM structure determination of a pLGIC increases our confidence that the structures are snapshots of functional states.

PubMed: 40668221
DOI: 10.7554/eLife.106728

実験手法

ELECTRON MICROSCOPY (3.4 Å)