9N6C

Structure of the Retron IA Complex without the HNH Nuclease

9N6C の概要

• エントリーDOI: 10.2210/pdb9n6c/pdb
• EMDBエントリー: 49056
• 分子名称: AAA family ATPase, RNA-directed DNA polymerase, Retron IA msDNA, ... (5 entities in total)
• 機能のキーワード: retron, ia, immune, transferase-dna-rna complex, transferase/dna/rna
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 339736.63

構造登録者

Burman, N.,Thomas-George, J.,Wilkinson, R.,Wiedenheft, B. (登録日: 2025-02-05, 公開日: 2025-04-23, 最終更新日: 2025-11-12)

主引用文献

George, J.T.,Burman, N.,Wilkinson, R.A.,de Silva, S.,McKelvey-Pham, Q.,Buyukyoruk, M.,Dale, A.,Landman, H.,Graham, A.B.,DeLuca, S.Z.,Wiedenheft, B.
Structural basis of antiphage defence by an ATPase-associated reverse transcriptase.
Nat Commun, 16:8459-8459, 2025

PubMed Abstract: Reverse transcriptases (RTs) have well-established roles in the replication and spread of retroviruses and retrotransposons. However, recent evidence suggests that RTs have been conscripted by cells for diverse roles in antiviral defence. Here we determine structures of a type I-A retron, which explain how RNA, DNA, RT, HNH-nuclease and four molecules of a structure maintenance of chromosome (SMC)-family ATPase assemble into a 364 kDa complex that provides phage defence. We show that phage-encoded nucleases trigger degradation of the retron-associated DNA, leading to activation of the HNH nuclease. The HNH nuclease cleaves tRNA, stalling protein synthesis and arresting viral replication. Taken together, these data reveal diverse and paradoxical roles for RTs in the perpetuation and elimination of genetic parasites.

PubMed: 41006229
DOI: 10.1038/s41467-025-63285-6

実験手法

ELECTRON MICROSCOPY (2.99 Å)