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9N4Y

CryoEM structure of a filamentous soluble pyridine nucleotide transhydrogenase

9N4Y の概要
エントリーDOI10.2210/pdb9n4y/pdb
EMDBエントリー48915
分子名称Soluble pyridine nucleotide transhydrogenase, FLAVIN-ADENINE DINUCLEOTIDE (2 entities in total)
機能のキーワードtranshydrogenase, flavoprotein
由来する生物種Azotobacter vinelandii
タンパク質・核酸の鎖数14
化学式量合計730484.72
構造登録者
Warmack, R.A. (登録日: 2025-02-03, 公開日: 2025-07-16, 最終更新日: 2025-09-17)
主引用文献Shen, Y.,Maggiolo, A.O.,Zhang, T.,Warmack, R.A.
CryoEM-enabled visual proteomics reveals de novo structures of oligomeric protein complexes.
Structure, 33:1484-, 2025
Cited by
PubMed Abstract: Single particle cryoelectron microscopy (cryoEM) and cryoelectron tomography (cryoET) are powerful methods for unveiling unique and functionally relevant structural states. Aided by mass spectrometry and machine learning, they promise to facilitate the visual exploration of proteomes. Leveraging visual proteomics, we interrogate structures isolated from a complex cellular milieu by cryoEM to identify and classify molecular structures and complexes de novo. By comparing three automated model building programs, CryoID, DeepTracer, and ModelAngelo, we determine the identity of six distinct oligomeric protein complexes from partially purified extracts of the nitrogen-fixing bacterium Azotobacter vinelandii using both anaerobic and aerobic cryoEM, including two original oligomeric structures. Overall, by allowing the study of near-native oligomeric protein states, cryoEM-enabled visual proteomics reveals unique structures that correspond to relevant species observed in situ.
PubMed: 40664216
DOI: 10.1016/j.str.2025.06.007
主引用文献が同じPDBエントリー
実験手法
ELECTRON MICROSCOPY (3.7 Å)
構造検証レポート
Validation report summary of 9n4y
検証レポート(詳細版)ダウンロードをダウンロード

243531

件を2025-10-22に公開中

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