9N0Y

PP2A-B55 Holoenzyme with Eya3

9N0Y の概要

• エントリーDOI: 10.2210/pdb9n0y/pdb
• EMDBエントリー: 48798
• 分子名称: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform, Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform, Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, ... (4 entities in total)
• 機能のキーワード: ser/thr phosphatase, complex, myc stabilization, oncoprotein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 215413.99

構造登録者

Shi, S.,Li, X.,Alderman, C.,Zhao, R. (登録日: 2025-01-24, 公開日: 2025-06-18, 最終更新日: 2025-07-09)

主引用文献

Shi, S.,Li, X.,Alderman, C.,Wick, L.,Huang, W.,Foulon, N.,Zhang, L.,Rossi, J.,Hu, W.,Cui, S.,Zheng, H.,Taylor, D.J.,Ford, H.L.,Zhao, R.
Cryo-EM structures reveal the PP2A-B55 alpha and Eya3 interaction that can be disrupted by a peptide inhibitor.
J.Biol.Chem., 301:110287-110287, 2025

PubMed Abstract: We have previously shown that Eya3 recruits PP2A-B55α to dephosphorylate pT58 on Myc, increasing Myc stability and enhancing primary tumor growth of triple-negative breast cancer (TNBC). However, the molecular details of how Eya3 recruits PP2A-B55α remain unclear. Here, we determined the cryo-EM structures of PP2A-B55α bound with Eya3, with an inhibitory peptide B55i, and in its unbound state. These studies demonstrate that Eya3 binds B55α through an extended peptide in the N-terminal domain of Eya3. The Eya3 peptide, PP2A-B55α substrates, and protein-peptide inhibitors including B55i bind to a similar area on the B55α surface, but the molecular details of the binding differ. We further demonstrated that the B55i peptide inhibits the B55α and Eya3 interaction in vitro. The B55i peptide expressed on a plasmid increases Myc pT58 and decreases Myc protein levels in TNBC cells, suggesting the potential of B55i or similar peptides as therapies for TNBC.

PubMed: 40414499
DOI: 10.1016/j.jbc.2025.110287

実験手法

ELECTRON MICROSCOPY (3.71 Å)