9N0W

HTLV-1 Gag capsid from immature particles

9N0W の概要

• エントリーDOI: 10.2210/pdb9n0w/pdb
• EMDBエントリー: 48795 48796
• 分子名称: Gag protein (1 entity in total)
• 機能のキーワード: complex, virus, lattice, viral protein
• 由来する生物種: Human T-cell leukemia virus type I
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 64305.55

構造登録者

Arndt, W.G.,Ramezani, A.,Chen, B.,Perilla, J.R.,Zhang, W.,Mansky, L.M. (登録日: 2025-01-24, 公開日: 2025-12-24, 最終更新日: 2026-01-21)

主引用文献

Arndt, W.G.,Ramezani, A.,Talledge, N.,Yu, G.,Yang, H.,Chen, B.,Perilla, J.R.,Zhang, W.,Mansky, L.M.
High-resolution analysis of the human T-cell leukemia virus capsid protein reveals insights into immature particle morphology.
Nat Commun, 17:444-444, 2025

PubMed Abstract: Infection with human T-cell leukemia virus type 1 (HTLV-1) can result in adult T-cell leukemia/lymphoma and HTLV-1 associated-myelopathy/tropical spastic paraparesis. The Gag polyprotein - the major structural protein - is crucial for driving virus particle assembly, with the capsid (CA) domain as the key determinant for Gag multimerization. Here, we characterize the immature CA lattice from immature virus particles by using cryo-electron microscopy and tomography (cryo-EM/ET). We report resolving the immature CA lattice to 3.4 Å resolution by single particle analysis (SPA). Our reconstruction reveals that the lattice is stabilized through a trimeric NTD inter-hexamer interface and a dimeric CTD inter-hexamer interface. Further analysis by cryo-ET reveals clear heterogeneity, notably the varying lattice curvatures and the varying distances from the CA layer to the membrane. Intriguingly, inositol hexakisphosphate (IP6) is dispensable for HTLV-1 immature particle assembly and proper immature lattice formation. These observations provide deeper insights into the molecular basis of HTLV-1 immature particle morphology as well as aid in revealing therapeutic targets.

PubMed: 41381513
DOI: 10.1038/s41467-025-67129-1

実験手法

ELECTRON MICROSCOPY (3.44 Å)