9MVV
Pfs230 (D9-D14) with Pfs48/45 of the endogenous Pfs230-Pfs48/45 complex
Summary for 9MVV
| Entry DOI | 10.2210/pdb9mvv/pdb |
| EMDB information | 48673 |
| Descriptor | Gametocyte surface protein P230, Gametocyte surface protein P45/48 (2 entities in total) |
| Functional Keywords | 6-cysteine protein, plasmodium falciparum, malaria transmission, protein binding |
| Biological source | Plasmodium falciparum (malaria parasite P. falciparum) More |
| Total number of polymer chains | 2 |
| Total formula weight | 412207.81 |
| Authors | Dietrich, M.H.,Glukhova, A.,Shakeel, S.,Tham, W.H. (deposition date: 2025-01-16, release date: 2025-07-30, Last modification date: 2025-09-24) |
| Primary citation | Dietrich, M.H.,Chmielewski, J.,Chan, L.J.,Tan, L.L.,Adair, A.,Lyons, F.M.T.,Gabriela, M.,Lopaticki, S.,Dite, T.A.,Dagley, L.F.,Pazzagli, L.,Gupta, P.,Kamil, M.,Vaughan, A.M.,Rojrung, R.,Abraham, A.,Mazhari, R.,Longley, R.J.,Zeglinski, K.,Gouil, Q.,Mueller, I.,Fabb, S.A.,Shandre-Mugan, R.,Pouton, C.W.,Glukhova, A.,Shakeel, S.,Tham, W.H. Cryo-EM structure of endogenous Plasmodium falciparum Pfs230 and Pfs48/45 fertilization complex. Science, 389:eady0241-eady0241, 2025 Cited by PubMed Abstract: Malaria parasite fertilization occurs in the midgut of a female mosquito. Blocking fertilization within the mosquito can prevent malaria transmission. Pfs230 and Pfs48/45 are critical for male fertility and transmission of the malaria parasite. They form a core fertilization complex, but it is unknown how they interact. We determined a cryo-electron microscopy structure of endogenous Pfs230-Pfs48/45 complex showing that Pfs48/45 interacts with Pfs230 domains 13 and 14. Transgenic parasite lines with these domains removed were defective in Pfs230 gamete localization and showed reduced oocyst formation. Nanobodies against domains 13 and 14 inhibited Pfs230-Pfs48/45 complex formation, reduced transmission and structural analyses revealed their epitopes. These Pfs230 domains were targets of naturally acquired immunity and immune sera from mRNA-lipid nanoparticle immunizations blocked parasite transmission. PubMed: 40743371DOI: 10.1126/science.ady0241 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.36 Å) |
Structure validation
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