9MTY

Structure of TIGR-TasR in complex with tigRNA and target DNA after DNA cleavage

9MTY の概要

• エントリーDOI: 10.2210/pdb9mty/pdb
• EMDBエントリー: 48616
• 分子名称: Transposase IS116/IS110/IS902 C-terminal domain-containing protein, tigRNA, Target DNA, spacer A targeted strand, 5' of cut, ... (7 entities in total)
• 機能のキーワード: nuclease, rna-guided, nop domain, dna-binding protein, dna-rna hybrid, dna binding protein-rna-dna complex, dna binding protein/rna/dna
• 由来する生物種: Thermoproteota archaeon; 詳細
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 125026.63

構造登録者

Wilkinson, M.E.,Zhang, F. (登録日: 2025-01-12, 公開日: 2025-03-05, 最終更新日: 2025-05-14)

主引用文献

Faure, G.,Saito, M.,Wilkinson, M.E.,Quinones-Olvera, N.,Xu, P.,Flam-Shepherd, D.,Kim, S.,Reddy, N.,Zhu, S.,Evgeniou, L.,Koonin, E.V.,Macrae, R.K.,Zhang, F.
TIGR-Tas: A family of modular RNA-guided DNA-targeting systems in prokaryotes and their viruses.
Science, 388:eadv9789-eadv9789, 2025

PubMed Abstract: RNA-guided systems provide remarkable versatility, enabling diverse biological functions. Through iterative structural and sequence homology-based mining starting with a guide RNA-interaction domain of Cas9, we identified a family of RNA-guided DNA-targeting proteins in phage and parasitic bacteria. Each system consists of a tandem interspaced guide RNA (TIGR) array and a TIGR-associated (Tas) protein containing a nucleolar protein (Nop) domain, sometimes fused to HNH (TasH)- or RuvC (TasR)-nuclease domains. We show that TIGR arrays are processed into 36-nucleotide RNAs (tigRNAs) that direct sequence-specific DNA binding through a tandem-spacer targeting mechanism. TasR can be reprogrammed for precise DNA cleavage, including in human cells. The structure of TasR reveals striking similarities to box C/D small nucleolar ribonucleoproteins and IS110 RNA-guided transposases, providing insights into the evolution of diverse RNA-guided systems.

PubMed: 40014690
DOI: 10.1126/science.adv9789

実験手法

ELECTRON MICROSCOPY (3.05 Å)