9MQS

CryoEM Structure of the Candida albicans Group I Intron-GMP Complex

9MQS の概要

• エントリーDOI: 10.2210/pdb9mqs/pdb
• EMDBエントリー: 48538
• 分子名称: RNA (332-MER), CALCIUM ION, POTASSIUM ION, ... (5 entities in total)
• 機能のキーワード: intron, group i, splicing, rna
• 由来する生物種: Candida albicans
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 107409.93

構造登録者

Chung, K.,Xu, L.,Liu, T.,Pyle, A. (登録日: 2025-01-06, 公開日: 2025-04-30, 最終更新日: 2025-05-28)

主引用文献

Liu, T.,Xu, L.,Chung, K.,Sisto, L.J.,Hwang, J.,Zhang, C.,Van Zandt, M.C.,Pyle, A.M.
Molecular insights into de novo small-molecule recognition by an intron RNA structure.
Proc.Natl.Acad.Sci.USA, 122:e2502425122-e2502425122, 2025

PubMed Abstract: Despite the promise of vastly expanding the druggable genome, rational design of RNA-targeting ligands remains challenging as it requires the rapid identification of hits and visualization of the resulting cocomplexes for guiding optimization. Here, we leveraged high-throughput screening, medicinal chemistry, and structural biology to identify a de novo splicing inhibitor against a large and highly folded fungal group I intron. High-resolution cryoEM structures of the intron in different liganded states not only reveal molecular interactions that rationalize experimental structure-activity relationship but also shed light on a unique strategy whereby RNA-associated metal ions and RNA conformation exhibit exceptional plasticity in response to small-molecule binding. This study reveals general principles that govern RNA-ligand recognition, the interplay between chemical bonding specificity, and dynamic responses within an RNA target.

PubMed: 40339124
DOI: 10.1073/pnas.2502425122

実験手法

ELECTRON MICROSCOPY (3.1 Å)