9MP2

Fatty Acid Binding Protein 4 (FABP4) Complexed with Perfluorohexadecanoic Acid (PFHxDA)

これはPDB形式変換不可エントリーです。

9MP2 の概要

• エントリーDOI: 10.2210/pdb9mp2/pdb
• 分子名称: Fatty acid-binding protein, adipocyte, hentriacontafluorohexadecanoic acid (3 entities in total)
• 機能のキーワード: pfas, hydrophobic, beta-barrel, adipocyte, lipid binding protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15749.23

構造登録者

Birchfield, A.B.,Musayev, F.N.,Fuglestad, B. (登録日: 2024-12-30, 公開日: 2025-06-11, 最終更新日: 2025-07-09)

主引用文献

Birchfield, A.S.,Musayev, F.N.,Castillo, A.J.,Zorn, G.,Fuglestad, B.
Broad PFAS Binding with Fatty Acid Binding Protein 4 Is Enabled by Variable Binding Modes.
Jacs Au, 5:2469-2474, 2025

PubMed Abstract: Per- and polyfluoroalkyl substances (PFAS) are ubiquitous pollutants that bioaccumulate in wildlife and humans, yet the molecular basis of their protein interactions remains poorly understood. Here, we show that human adipocyte fatty acid-binding protein 4 (FABP4) can bind a diverse array of PFAS, including next-generation replacements for legacy chemicals and longer-chain perfluorocarboxylic acids. Shorter-chain PFAS, although weaker binders, still displayed measurable affinities, surpassing those of their nonfluorinated analogs. We determined crystal structures of FABP4 bound to perfluorooctanoic acid (PFOA), perfluorodecanoic acid (PFDA), and perfluorohexadecanoic acid (PFHxDA), revealing three distinct binding modes. Notably, PFOA binds in two separate sites, and two distinct conformations define single-ligand binding of PFDA and PFHxDA. These arrangements enhance hydrophobic interactions within the binding cavity and likely explain the low micromolar dissociation constants observed in fluorescence competition assays. Our findings underscore the critical roles of chain length, headgroup functionality, and protein conformation in PFAS-FABP4 interactions. Given the emerging implications of the role of FABP4 in endocrine function, even subtle PFAS-induced perturbations could affect metabolic regulation and disease risk. Overall, this work highlights the value of direct structural and biochemical insights into PFAS-FABP4 interactions and paves the way for future research on PFAS transport and toxicological outcomes.

PubMed: 40575325
DOI: 10.1021/jacsau.5c00504

実験手法

X-RAY DIFFRACTION (1.35 Å)